MURINE CELLS EXPRESSING AN HLA MOLECULE ARE SPECIFICALLY LYSED BY HLA-RESTRICTED ANTIVIRAL HUMAN T-CELLS
MURINE CELLS EXPRESSING AN HLA MOLECULE ARE SPECIFICALLY LYSED BY HLA-RESTRICTED ANTIVIRAL HUMAN T-CELLS
复制标题
DOI：
10.1038/319153a0
复制
发表时间：
1986-01-09
期刊：
影响因子：
64.8
通讯作者：
LEVY, JP
中科院分区：
文献类型：
--
作者：
GOMARD, E;BEGUE, B;LEVY, JP
HLA Ⅰ类分子是同种异体特异性溶细胞性T淋巴细胞（CTL）在移植排斥反应中的作用靶点，是抗病毒CTL与病毒感染细胞相互作用的限制性因子。在缺乏其他人类分子的情况下仅表达一种HLA的细胞将为研究这些分子的功能提供一个显着的模型。然而，用人基因转染的HLA+鼠细胞1 - 5通常不被同种异体特异性人CTL 5 -8裂解，这归因于HLA表达不足、缺乏人β2-微球蛋白、HLA分子改变或鼠细胞中缺乏人T8或LFA 1分子的受体6 -8。在这里，我们报告，为第一次，特异性裂解病毒感染的HLA+小鼠细胞的HLA限制性抗病毒的人CTL。因此，这些鼠细胞构成了研究HLA分子作用的极好模型。
Class I HLA (histocompatibility locus antigen) molecules are the targets of allospecific cytolytic T lymphocytes (CTL) in graft rejection, and constitute the restricting elements necessary for the interaction between antiviral CTL and virus-infected cells. Cells expressing only one HLA in the absence of other human molecules would provide a remarkable model for studying the function of these molecules. However, HLA+murine cells transfected with human genes1–5are generally not lysed by allospecific human CTL5–8, and this is ascribed to insufficient HLA expression, lack of humanβ2-microglobulin, alteration of HLA molecules or absence of receptors for human T8 or LFA1 molecules in murine cells6–8. Here we report, for the first time, the specific lysis of virus-infected HLA+murine cells by HLA-restricted antiviral human CTL. Therefore, these murine cells constitute an excellent model for studying the role of HLA molecules.