In vivo near‐infrared monitoring of nitric oxide production and tissue oxygen sufficiency in rat liver allografts during the acute rejection reaction
In vivo near‐infrared monitoring of nitric oxide production and tissue oxygen sufficiency in rat liver allografts during the acute rejection reaction
复制标题
急性排斥反应期间大鼠同种异体肝移植物中一氧化氮产生和组织氧充足度的体内近红外监测
DOI：
10.1111/j.1432-2277.1994.tb01439.x
复制
发表时间：
1994
影响因子：
3.1
通讯作者：
M. Kanashiro
中科院分区：
文献类型：
--
作者：
H. Ohdan;Y. Fukuda;K. Dohi;S. Suzuki;H. Amemiya;M. Kanashiro
摘要我们建立了一种新的体内近红外（NIR）光谱技术，可以估计同种免疫反应期间活器官中一氧化氮（NO）的产生和组织氧充足度。本研究的目的是评估这种技术的潜力监测排斥反应利用大鼠原位肝移植模型无动脉化。通过使用该方法定量移植肝脏中亚硝基血红蛋白、氧合血红蛋白和氧化型细胞色素氧化酶的相对变化。同种异体移植物中的亚硝基血红蛋白在排斥反应发生时升高，当通过给予15-脱氧精胍菌素治疗排斥反应时受到抑制。氧合血红蛋白和氧化型细胞色素氧化酶降低与组织学确定的实质疾病一致。结果表明，活体近红外光谱新技术可以同时评价肝移植术后的免疫反应和移植物功能。
Abstract We established a new technique of in vivo near‐infrared (NIR) spectroscopy that can estimate both nitric oxide (NO) production and tissue oxygen sufficiency in living organs during the alloimmune response. The present study was aimed at evaluating the potential of this technique for monitoring the rejection response utilizing the rat model of orthotopic liver transplantation without arterialization. The relative changes of nitrosyl‐hemoglobin, oxyhemoglobin and oxidized‐cytochromeoxidase in the graft livers were quantified by use of this method. Nitrosyl‐hemoglobin in the allogenic grafts was elevated at the onset of the rejection response and was suppressed when the rejection reaction was treated by the administration of 15‐deoxyspergualin. Oxy‐hemoglobin and oxidized‐cytochromeoxidase were decreased in accordance with parenchymal disorder determined histologically. These results demonstrated that the new technique of in vivo NIR spectroscopy can assess simultaneously both the immune respons and graft function after liver transplantation.
登录
查看更多内容
影响因子：
3.8
作者：
Jan M. Langrehr;Rosemary A. Hoffman;T. Billiar;Kenneth K. Lee;Wolfgang H. Schraut;Simmons Rl
通讯作者：
Jan M. Langrehr;Rosemary A. Hoffman;T. Billiar;Kenneth K. Lee;Wolfgang H. Schraut;Simmons Rl
通过电子顺磁共振波谱法检测大鼠小肠移植后排斥反应和移植物抗宿主病期间的一氧化氮。
影响因子：
3.8
作者：
Langrehr,JM;Muller,AR;Bergonia,HA;Jacob,TD;Lee,TK;Schraut,WH;LancasterJr,JR;Hoffman,RA;Simmons,RL
通讯作者：
Simmons,RL
影响因子：
2.9
作者：
MARLETTA, MA;YOON, PS;WISHNOK, JS
通讯作者：
WISHNOK, JS
尿硝酸盐排泄与小鼠巨噬细胞活化相关。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Granger,DL;HibbsJr,JB;Broadnax,LM
通讯作者：
Broadnax,LM