Contribution of gut bacteria to digestion and development of the velvetbean caterpillar, Anticarsia gemmatalis.
Contribution of gut bacteria to digestion and development of the velvetbean caterpillar, Anticarsia gemmatalis.
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DOI：
10.1016/j.jinsphys.2008.10.017
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发表时间：
2009-03
影响因子：
2.2
通讯作者：
L. E. Visôtto;M. G. A. Oliveira;R. Guedes;A. Ribon;P. I. V. Good-God
中科院分区：
文献类型：
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作者：
L. E. Visôtto;M. G. A. Oliveira;R. Guedes;A. Ribon;P. I. V. Good-God
用22种抗生素对5龄绒豆毛虫肠道匀浆中的细菌菌落进行了抗生素敏感性试验。抗生素四环素的效果最好，其次是氯霉素。四环素对菌落形成单位的抑制作用也高于氯霉素，因此，在增加饲料浓度的情况下向毛虫提供四环素，以评估肠道细菌对其消化和发育的贡献。四环素显著抑制蛋白酶（一般）、丝氨酸蛋白酶和脂肪酶的活性。浓度抑制曲线成功地建立了四环素，这种抗生素是有效的抑制它们，特别是丝氨酸蛋白酶，这表明肠道细菌可能显着有助于与脂质和主要蛋白质消化的绒豆毛虫。增加四环素的饲料浓度仅导致昆虫死亡率的轻度增加（约100%）。随着四环素浓度的增加，存活的昆虫表现出更快的发育（≤4天）和更高的蛹重（<0.04mg）。因此，四环素抑制的肠道细菌似乎并没有在绒豆毛虫的生存和发展中发挥至关重要的作用，但可能是重要的，在这种害虫物种的适应富含蛋白酶抑制剂的主机，如大豆。
Bacteria colonies from gut homogenates of fifth instar velvetbean caterpillars (Lepidoptera: Noctuidae) were subjected to antibiotic sensitivity experiments using discs containing 22 antibiotics. The antibiotic tetracycline provided the best results, followed by chloramphenicol. Tetracycline also provided higher inhibition of colony forming units than chloramphenicol and was therefore provided to the caterpillars in increasing diet concentrations to assess the contribution of gut bacteria to their digestion and development. The activity of proteases (general), serine-proteinases and lipases were significantly suppressed by tetracycline. Concentration–inhibition curves were successfully established for tetracycline and this antibiotic was effective in suppressing them, particularly serine-proteinases, suggesting that gut bacteria may significantly contribute with lipid- and mainly protein-digestion in velvetbean caterpillars. Increased diet concentrations of tetracycline led only to mild increase in insect mortality (ca. 20%), with the surviving insects showing faster development (≤4 days) and higher pupa weight (<0.04mg) with increased concentrations of tetracycline. Therefore, the gut bacteria inhibited by tetracycline does not seem to play a crucial role in the survival and development of the velvetbean caterpillar, but may be important in the adaptation of this pest species to hosts rich in protease inhibitors, such as soybean.