INITIATION OF SPERMIOGENESIS IN C-ELEGANS - A PHARMACOLOGICAL AND GENETIC-ANALYSIS
INITIATION OF SPERMIOGENESIS IN C-ELEGANS - A PHARMACOLOGICAL AND GENETIC-ANALYSIS
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DOI：
10.1016/0012-1606(89)90088-2
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发表时间：
1989-07-01
影响因子：
2.7
通讯作者：
WARD, S
中科院分区：
文献类型：
--
作者：
SHAKES, DC;WARD, S
秀丽隐杆线虫的精子发生过程包括将球形无柄精子细胞转变为两极爬行精子。在雄性中，精子发生是通过交配诱导的，而在两性人中，精子发生在第一个卵母细胞受精之前发生。或者，也可以在体外用莫能菌素、三乙醇胺或链霉素酶处理来诱导精子生成。用钙调素抑制剂三氟拉嗪、氯丙嗪或W7在体外也能诱导精子生成，在20微米时效果一半最大。在最初激活时，精子细胞延伸又长又细的棘突并进行广泛的细胞运动。最终，通过重组这些尖峰中的一个或多个，形成一个单一的移动伪足。这些短暂的尖峰在体外可以通过在精子细胞第一次形成尖峰时去除三乙醇胺来延长。来自spe-8和spe-12精子发生缺陷(Spe)突变体的精子细胞在体内被男性精子激活剂激活，而不是两性精子激活剂。在体外，突变的精子细胞用链霉蛋白酶阻止精子发生在穗状期，但如果随后或最初用莫尼辛或三乙醇胺处理，则形成正常精子。我们提出了一个精子发生的模型，其中突变缺陷和药物的作用相对于彼此是有序的。
Spermiogensis in Caenorhabditis elegans involves the conversion of spherical, sessile spermatids into bipolar, crawling spermatozoa. In males, spermiogenesis is induced by mating, while in hermaphrodites, spermiogenesis occurs before the first oocytes ar fertilized. Alternatively, spermiogenesis can be induced in vitro by treatemtn with monensin, triethanolamine, or pronase. Treatment with the calmodulin inhibitors, trifluoperazine, chlorpromazine, or W7, also induces spermiogenesis in vitro with a half maximal effect at 20 .mu.M. Upon initial activation, spermatids extend long, thin spikes and undergo extensive cellular movements. Eventually, a single motile pseudopod forms through the restructuring of one or more of these spikes. These transient spikes can be prolonged in vitro by removing triethanolamine as soon as the spermatids first form spikes. Spermatids from spe-8 and spe-12 spermatogenesis-defective (spe) mutants activate in vivo with male but not hermaphrodite sperm activator. In vitro, the mutant spermatids arrest spermiogenesis at the spike stage with pronase, but form normal spermatozoa if subsequently or initially treated with monesin or triethanolamine. We present a model of spermiogenesis in which the mutant defects and the action of the pharmacological agents are ordered relative to one another.