Another formula for calculating the gene change rate in real-time RT-PCR
Another formula for calculating the gene change rate in real-time RT-PCR
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DOI：
10.1007/s11033-008-9430-1
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发表时间：
2009-11-01
影响因子：
2.8
通讯作者：
Yang, Jicheng
中科院分区：
文献类型：
--
作者：
Chang, Shujian;Chen, Weichang;Yang, Jicheng
相对定量实时荧光定量PCR已成为分子生物学研究中研究微量基因表达的一种常规方法。实时荧光定量PCR的相对定量数据分析有一些数学模型，公式2^(-ΔΔCT)是最常用的方法之一。在本文中，我们将提出另一个直接计算基因表达变化率（R(c)）的公式，并举例比较该模型与2^(-ΔΔCT)的应用。
Relative quantification real-time PCR has become a routine method in molecular biology research to study the small amount gene expression. There are a few mathematical models for relative quantification data analysis in real-time PCR, and the formula 2(-a dagger a dagger CT) is one of the most frequently used method. In this paper, we are to present another equation which directly calculates the change rate (R (c)) of gene expression, and give a example to compare the application of this model with 2(-a dagger a dagger CT).