Chronic Intermittent Hypobaric Hypoxia Ameliorates Ischemia/Reperfusion-Induced Calcium Overload in Heart via Na+/Ca2+ Exchanger in Developing Rats
Chronic Intermittent Hypobaric Hypoxia Ameliorates Ischemia/Reperfusion-Induced Calcium Overload in Heart via Na+/Ca2+ Exchanger in Developing Rats
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DOI：
10.1159/000363001
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发表时间：
2014-01-01
影响因子：
--
通讯作者：
Zhang, Yi
中科院分区：
文献类型：
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作者：
Ma, Hui-Jie;Li, Qian;Zhang, Yi
背景/目的：慢性间歇性低压缺氧(CIHH)可保护心脏免受缺血/再灌注(I/R)损伤。本研究探讨了钙稳态机制及Na+/Ca~(2+)交换器(NCX)在CIHH对发育大鼠心脏保护作用中的作用。方法：新生雄性大鼠在模拟海拔3000米的低压舱中连续42天接受CIHH治疗或不治疗(对照组)。分别于缺血30min和再灌流60min后评价离体心功能。测定心肌梗死面积、心肌细胞内钙离子浓度([Ca~(2+)]_i)、心肌细胞Na~+-Ca~(2+)交换电流(I-Na/Ca)和肌膜NCX1蛋白水平。结果：与对照组比较，CIHH大鼠I/R后心功能恢复明显改善，心肌梗死面积缩小(P<0.05)。
Background/Aims: Chronic intermittent hypobaric hypoxia (CIHH) protects the heart against ischemia/reperfusion (I/R) injury. This study investigated the calcium homeostasis mechanism and the role of Na+/Ca2+ exchanger (NCX) in the cardiac protective effect of CIHH in developing rats. Methods: Neonatal male rats received CIHH treatment or no treatment (control) in a hypobaric chamber simulating 3000-meter altitude for 42 days. The left ventricular function of isolated hearts was evaluated after 30 minutes of ischemia and 60 minutes of reperfusion. Myocardial infarct size, intracellular Ca2+ concentration ([Ca2+],), Na+-Ca2+ exchanger currents (I-Na/Ca) in ventricular myocytes, and NCX1 protein level in the sarcolemmal membrane were determined. Results: The recovery of cardiac function after I/R was improved, with the myocardial infarct size reduced, in CIHH rats compared with control rats (p