MAP kinase subcellular localization controls both pattern and proliferation in the developing Drosophila wing
MAP kinase subcellular localization controls both pattern and proliferation in the developing Drosophila wing
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DOI：
10.1242/dev.02168
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发表时间：
2006-01-01
期刊：
影响因子：
4.6
通讯作者：
Moses, K
中科院分区：
文献类型：
--
作者：
Marenda, DR;Vrailas, AD;Moses, K
有丝分裂原活化蛋白激酶（MAPK）磷酸化细胞质和细胞核中的靶蛋白，并且MAPK的亚细胞定位与所产生的细胞应答之间存在强相关性。一般认为MAPK磷酸化后总是伴随着快速核转位。然而，我们和其他人发现MAPK磷酸化并不总是足以在体内进行核转位。在发育中的果蝇翅膀中，MAPK介导的信号传导对于图案形成和细胞增殖都是必需的，尽管这种差异控制的机制还没有完全理解。在这里，我们表明，磷酸化MAPK（pMAPK）是在细胞质中举行的分化幼虫和蛹翼静脉细胞，我们表明，这种细胞质举行静脉细胞的命运。与此同时，我们发现MAPK确实进入了其他翼细胞的细胞核，在那里它促进了细胞增殖。我们提出了一种新的Ras通路在果蝇和我们的研究结果表明，MAPK磷酸化的机制，可以信号两种不同的细胞结果（分化与增殖）的基础上的MAPK的亚细胞定位。
Mitogen-activated protein kinases (MAPKs) phosphorylate target proteins in both the cytoplasm and nucleus, and a strong correlation exists between the subcellular localization of MAPK and resulting cellular responses. It was thought that MAPK phosphorylation was always followed by rapid nuclear translocation. However, we and others have found that MAPK phosphorylation is not always sufficient for nuclear translocation in vivo. in the developing Drosophila wing, MAPK-mediated signaling is required both for patterning and for cell proliferation, although the mechanism of this differential control is not fully understood. Here, we show that phosphorylated MAPK (pMAPK) is held in the cytoplasm in differentiating larval and pupal wing vein cells, and we show that this cytoplasmic hold is required for vein cell fate. At the same time, we show that MAPK does move into the nucleus of other wing cells where it promotes cell proliferation. We propose a novel Ras pathway bifurcation in Drosophila and our results suggest a mechanism by which MAPK phosphorylation can signal two different cellular outcomes (differentiation versus proliferation) based on the subcellular localization of MAPK.