Rapid and simple quantitative measurement of alpha-fetoprotein by combining immunochromatographic strip test and artificial neural network image analysis system.
Rapid and simple quantitative measurement of alpha-fetoprotein by combining immunochromatographic strip test and artificial neural network image analysis system.
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DOI：
10.1016/j.cccn.2004.05.010
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发表时间：
2004-10
期刊：
影响因子：
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通讯作者：
L. Chuang;Jeng-Yang Hwang;Hen-Chang Chang-Hen-Chang-Chang-3261940;F. Chang;S. Jong
中科院分区：
文献类型：
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作者：
L. Chuang;Jeng-Yang Hwang;Hen-Chang Chang-Hen-Chang-Chang-3261940;F. Chang;S. Jong
[背景]免疫层析定量试条比传统的定性或半定量试条提供了更多的信息，为临床诊断提供了更多的信息。方法将免疫层析试条与人工神经网络图像分析系统相结合，构建了人血清甲胎蛋白(α)检测系统。ICS试验完成后，用IAIS软件进行分析，即可得到甲胎蛋白浓度。所需设备包括商用半定量甲胎蛋白试纸、光学扫描仪、离心机(OPTION)、个人计算机和IAIS软件。结果IAIS测定的血清甲胎蛋白浓度与放射免疫法测定的甲胎蛋白浓度高度相关(r=0.9971)。使用Bland-Altman分析，IAIS与RIA相比达到了临床可接受的一致性限度。以变异系数表示，该方法的批内精密度为1.50%，在244.4 ng/ml时为1.09%。结论建立的免疫层析定量试纸条法(IAIS)是一种简便、快速、可靠的血清AFP检测方法。只需简单的设备，IAIS就可以在实验室外进行，是门诊或临床点AFP检测的理想选择。
BackgroundQuantitative immunochromatographic strip (ICS) assay can facilitate clinical diagnosis by providing more information than traditional qualitative or semiquantitative strip assay.MethodsWe constructed a human serum α-fetoprotein (AFP) measurement system by combining semiquantitative ICS tests and artificial neural network (ANN) image analysis system [immunochromatographic strip analyzed by artificial neural network image analysis system (IAIS)]. After ICS tests completed, the AFP concentration can be obtained from analysis of IAIS software. The equipment required are commercial semiquantitative AFP dipstick, optical scanner, centrifuge (option), personal computer, and software of IAIS.ResultsThe serum AFP concentrations measured by IAIS were strongly correlated (r=0.9971) with that by RIA. Using Bland–Altman analysis, the IAIS achieved clinical acceptable limits of agreement in comparison with RIA. The within-run precision of IAIS, expressed as coefficients of variation (CV), at 81.7 ng/ml was 1.50% and, at 244.4 ng/ml, was 1.09%. The measurement of serum AFP by IAIS can be completed in 20 min.ConclusionsThe newly constructed quantitative immunochromatographic strip assay (IAIS) is a simple, rapid, and reliable method for serum AFP measurement. With the simple equipment required, the IAIS can be performed outside the laboratory and is ideal for outpatient or point-of-care AFP testing.