A STUDY OF THE MECHANISM OF PINOCYTOSIS
A STUDY OF THE MECHANISM OF PINOCYTOSIS
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DOI：
10.1016/0014-4827(58)90032-6
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发表时间：
1958-01-01
影响因子：
3.7
通讯作者：
BRANDT, PW
中科院分区：
文献类型：
--
作者：
BRANDT, PW
本文描述了胞饮作用过程中活的阿米巴（混沌）的形态。在连续流动压缩室中观察到了这些变化。用过碘酸希夫技术（PAS）和荧光抗体标记技术染色的切片与活体阿米巴的观察结果相关联。测试盐和蛋白质溶液诱导胞饮作用的活性。实验结果表明，盐和蛋白质在诱导胞饮中具有活性，而不带电荷的溶质则没有。蛋白质被证明是吸附到质膜之前，胞饮。PAS技术显示质膜形成胞饮泡壁。胞饮作用的机制，建议涉及的质膜上的张力和结构刚度的膜的减少的带电溶质的吸附。被削弱的膜通过其与可收缩的浆凝胶系统的附着点被拉入细胞质。
The morphology of living amebae (Chaos chaos) during pinocytosis is described. The amebae were observed in a continuous flow compression chamber. Sections stained with the Periodic Acid Schiff Technique (PAS) and fluorescent antibody labeling techniques were correlated with the observations on the living amebae. Solutions of salts and proteins were tested for their activity in inducing pinocytosis. Experimental results showed that salts and proteins were active in inducing pinocytosis while non-charged solutes were not. Proteins were shown to be adsorbed to the plasmalemma prior to pinocytosis. The plasmalemma was shown by PAS technique to form the wall of the pinocytosis vacuole. A mechanism for pinocytosis is suggested which relates adsorption of charged solutes on the plasmalemma to a decrease in the tension and structural rigidity of the membrane. The weakened membrane is drawn down into the cytoplasm by its points of attachment to the contractile plasmagel system.