Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions
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DOI：
10.1038/srep10734
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发表时间：
2015-06-01
影响因子：
4.6
通讯作者：
Svergun, Dmitri I.
中科院分区：
文献类型：
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作者：
Graewert, Melissa A.;Franke, Daniel;Svergun, Dmitri I.
小角x射线散射（SAXS）是一种越来越受欢迎的分析溶液中生物大分子结构的方法，但样品固有的多分散性往往阻碍了该方法的应用。我们开发了一种一体化系统，将在线样品成分分离与分离组分的平行生物物理和SAXS表征相结合。该系统与自动数据分析管道相结合，为在P12同步加速器光束线（EMBL/DESY, Hamburg）上研究困难样品提供了一种新颖的工具。
Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).