Biological activities of ACL-I and physicochemical properties of ACL-II, lectins isolated from the marine sponge Axinella corrugata
Biological activities of ACL-I and physicochemical properties of ACL-II, lectins isolated from the marine sponge Axinella corrugata
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DOI：
10.1016/j.cbpb.2012.01.001
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发表时间：
2012-04-01
影响因子：
2.2
通讯作者：
Vozari-Hampe, Magdolna M.
中科院分区：
文献类型：
--
作者：
Dresch, Roger R.;Lerner, Clea B.;Vozari-Hampe, Magdolna M.
来自海洋海绵 Axinella corrugata (ACL-II) 的凝集素 II 通过亲和色谱法在掺入聚丙烯酰胺凝胶的兔红细胞基质上纯化，然后在 Ultrogel AcA 44 柱上进行凝胶过滤。纯化的 ACL-II 是一种凝集素，Mr 分别为 80 kDa 和 78 kDa，通过 SDS-PAGE 和 Superose 12 HR 柱上的 FPLC 分别估算。 ACL-II 主要凝集天然兔红细胞，这种血凝活性与 Ca2+、Mg2+ 和 Mn2+ 无关，但受 D-半乳糖、几丁质和 N-乙酰衍生物（GalINAc 除外）的抑制。 ACL-II 在高达 65°C 的温度下稳定 30 分钟，在 pH 2 至 6 范围内稳定性更好。相反，ACL-I 显示出强烈的促有丝分裂和细胞毒作用。 (C) 2012 Elsevier Inc. 保留所有权利。
Lectin II from the marine sponge Axinella corrugata (ACL-II) was purified by affinity chromatography on rabbit erythrocytic stroma incorporated into a polyacrylamide gel, followed by gel filtration on Ultrogel AcA 44 column. Purified ACL-II is a lectin with an Mr of 80 kDa and 78 kDa, estimated by SDS-PAGE and by FPLC on Superose 12 HR column, respectively. ACL-II mainly agglutinates native rabbit erythrocytes and this hemagglutinating activity is independent of Ca2+, Mg2+ and Mn2+, but is inhibited by D-galactose, chitin and N-acetyl derivatives, with the exception of GaINAc. ACL-II is stable for up to 65 degrees C for 30 min, with a better stability at a pH range of 2 to 6. In contrast, ACL-I displays a strong mitogenic and cytotoxic effect. (C) 2012 Elsevier Inc. All rights reserved.