Stress-induced MAP kinase Hog1 is part of transcription activation complexes
Stress-induced MAP kinase Hog1 is part of transcription activation complexes
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DOI：
10.1016/s1097-2765(01)00221-0
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发表时间：
2001-04-01
期刊：
影响因子：
16
通讯作者：
Ammerer, G
中科院分区：
文献类型：
--
作者：
Alepuz, PM;Jovanovic, A;Ammerer, G
为了应对高渗环境，大多数真核细胞激活一个专门的丝裂原活化蛋白（MAP）激酶途径。In S.在酿酒酵母中，关键蛋白激酶Hog1协调各种致力于干旱适应和一般胁迫保护的基因的转录诱导。根据启动子背景，Hog1可以通过多种结构上不相关的转录因子发挥作用。使用染色质沉淀分析，我们发现激酶本身在应激反应期间与启动子区域紧密相连。这种相互作用依赖于应激介导的转录激活因子的存在。反过来，Hog1调节这些因子中至少一个的启动子关联。其他研究结果强调了Hog1构成上游激活复合物的一个组成部分的可能性，可能不仅针对激活剂，而且还针对一般转录机制的组成部分。
In response to hyperosmotic environments, most eukaryotic cells activate a specialized mitogen-activated protein (MAP) kinase pathway. In S. cerevisiae, the key protein kinase, Hog1, coordinates the transcriptional induction of a variety of genes devoted to osmoadaptation and general stress protection. Depending on the promoter context, Hog1 can function through a variety of structurally unrelated transcription factors. Using chromatin precipitation assays, we discovered that the kinase itself becomes intimately linked with promoter regions during stress responses. This interaction is dependent on the presence of stress-mediating transcriptional activators. In turn, Hog1 modulates promoter association of at least one of these factors. Additional findings highlight the possibility that Hog1 constitutes an integral part of the upstream activation complex, perhaps targeting not only the activator but also components of the general transcription machinery.