Reduced adolescent risk-assessment and lower nicotinic beta-2 expression in rats exposed to nicotine through lactation by forcedly drinking dams
Reduced adolescent risk-assessment and lower nicotinic beta-2 expression in rats exposed to nicotine through lactation by forcedly drinking dams
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DOI：
10.1016/j.neuroscience.2019.06.014
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发表时间：
2019-08-10
期刊：
影响因子：
3.3
通讯作者：
Adriani, Walter
中科院分区：
文献类型：
--
作者：
Faure, Alexis;Zoratto, Francesca;Adriani, Walter
很少有动物研究关注出生后尼古丁暴露的后果，特别是通过哺乳。我们最近的研究表明，被迫饮用尼古丁会提高母性的关爱，反而会在幼崽身上引发兴奋和压力。目前的工作旨在评估改变的产妇护理的具体贡献，与仅仅由于尼古丁影响的后遗症相比。两组与饮水控制坝进行比较：(i)自由选择坝（H20 + NIC组）从两个瓶子中饮用，瓶子中含有尼古丁或水；（ii）强迫水坝（NIC +NIC组）从两个瓶子中饮用，都含有尼古丁。我们之前已经证明尼古丁确实会转移到哺乳期的后代身上。关于青春期的行为后果，H20+ NIC和NIC+ NIC大鼠在熟悉的隔间上发现小说的速度都比对照组慢，而只有NIC+ NIC大鼠表现出与风险相关的回避和评估行为降低。成年后的大脑分析表明，在前额叶皮层，尼古丁本身降低了血清素，而母亲的过度护理降低了CHRN-B2基因的表达。总体而言，不可避免的尼古丁增强母性护理可能通过作用于前额叶CHRN-B2基因表达对后代觉醒产生影响。当目前的结果转化为人类非自愿暴露的后果时，我们提出，接受吸烟照顾者改变注意力的儿童可能会经历一种倾向于情感羞怯的神经行为发展。(c) 2019 ibroElsevier Ltd.出版。版权所有。
Few animal studies focus on consequences of nicotine postnatal exposure, particularly through lactation. We have recently shown that forced nicotine drinking elevates maternal care, paradoxically provoking arousal and stress in pups. Present work aimed to evaluate the specific contribution of altered maternal cares, compared to the sequelae merely due to nicotine effects. Two groups were compared to water-drinking control dams: (i) free-choice dams (H20 + NIC group) drinking from two bottles, containing either nicotine or water; (ii) forced dams (NIC +NIC group) drinking from two bottles, both containing nicotine. We previously demonstrated that nicotine was indeed transferred to the lactating offspring. Regarding behavioural consequences at adolescence, both H20+ NIC and NIC+ NIC rats were slower than controls in discovering a novel over a familiar compartment, whilst only NIC+ NIC rats exhibited reduced risk-related avoidance and assessment behaviour. Brain analyses at adulthood suggest that, in prefrontal cortex, nicotine per se reduced serotonin, while the maternal overcare reduced CHRN-B2 gene-expression. As a whole, unescapable nicotine-enhanced maternal care could have an impact on the offspring arousal by acting on prefrontal CHRN-B2 gene-expression. When present results are translated to consequences of nonvoluntary exposure in humans, we propose that children receiving altered attentions by a smoking caregiver might undergo a neuro-behavioural development biased towards emotional shyness. (C) 2019 IBRO. Published by Elsevier Ltd. All rights reserved.