USE OF LIGATED SEGMENTS OF RABBIT SMALL INTESTINE IN EXPERIMENTAL SHIGELLOSIS
USE OF LIGATED SEGMENTS OF RABBIT SMALL INTESTINE IN EXPERIMENTAL SHIGELLOSIS
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DOI：
10.1128/jb.89.3.803-809.1965
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发表时间：
1965-01-01
影响因子：
3.2
通讯作者：
HAYES, JR
中科院分区：
文献类型：
--
作者：
ARM, HG;FLOYD, TM;HAYES, JR
用最近分离的志贺氏菌菌株的肉汤培养物或静息细胞悬浮液接种活家兔结扎的小肠段，在12小时内通过渗出物的积累引起小肠段膨胀。炎症反应的组织学变化特征与人类细菌性痢疾相似。在接种2 × 1010个静息细胞的志贺氏菌数量明显增加之前，组织反应和渗出物的积累。志贺氏菌引起肠道反应的能力与任何特定的血清学组无关。与引起肠道反应有关的活性原理仅与含有活生物体的制剂有关。在人工培养基上培养时，最近分离的染色引起反应的能力迅速下降。从人的急性细菌性痢疾感染中分离出来后不久，冻干法无限期地保存了这种能力。指出了利用最近分离的志贺氏菌研究可能的发病机制的优点。在夏季，家兔小肠对志贺氏菌的活性具有抗性，未观察到阳性反应。使用兔小肠结扎段作为兔毒力指标；从人痢疾杆菌感染中分离出志贺氏菌后，在人工培养基上短时间培养对家兔的毒力有高度的相关性。
Inoculation of ligated segments of small intestine in living rabbits with broth cultures or resting-cell suspensions of recently isolated strains of Shigella caused distension of the segments by accumulation of exudate within 12 hr. Histological changes characteristic of an inflammatory response were similar to those of human bacil-lary dysentery. Tissue response and accumulation of exudate preceded demonstrable increase in numbers of shigellae inoculated as 2 x 1010 resting cells. Capability of shigellae to provoke intestinal response was not related to any particular serological group. The active principles concerned with eliciting intestinal response were associated only with preparations containing living organisms. Ability of recently isolated stains to elicit response diminished rapidly during culture on artificial media. The capability was preserved indefinitely by lyophilization soon after isolation from acute bacillary dysentery infections of man. Advantages of using shigellae recently isolated for investigating possible mechanisms of pathogenesis were indicated. During the summer months, the rabbit small intestine was refractory to the activity of shigellae, and positive responses were not observed. Use of ligated segments of rabbit small intestine qualified as an indicator of virulence for the rabbit; and, virulence for the rabbit showed a high degree of correlation with a short period of culture of shigellae on artificial media after isolation from human bacillary dysentery infections.