Silver carboxylate-TiO2/polydimethyl siloxane is a safe and effective antimicrobial with significant wound care potential.
Silver carboxylate-TiO2/polydimethyl siloxane is a safe and effective antimicrobial with significant wound care potential.
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羧酸银-TiO2/聚二甲基硅氧烷是一种安全有效的抗菌剂，具有显着的伤口护理潜力。
DOI：
10.1097/oi9.0000000000000299
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Garcia,DioscarisR
中科院分区：
文献类型：
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作者：
Allu,Sai;Whitaker,Colin;Stone,Benjamin;Vishwanath,Neel;Clippert,Drew;Jouffroy,Elia;Antoci,Valentin;Born,Christopher;Garcia,DioscarisR
方法：通过机构动物护理和使用委员会和IRB批准的方案，将AgCar应用于活尤卡坦猪皮肤，并对皮肤渗透进行组织学分析。石墨炉原子吸收光谱法（GFAAS）用于测定AgCar的洗脱。通过光密度生成剂量-反应曲线以评估效价。最后，使用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物测定来定量新制剂的细胞毒性。使用方差分析和事后Tukey检验对结果进行统计分析。结果：在动物模型中，羧酸银涂层在深层毛囊皮脂腺的水平上表现出深入渗透到上皮中。GFAAS测试证明羧酸银在96小时内的延长洗脱曲线，而不含二氧化钛-聚二甲基硅氧烷基质的100%银在48小时内完全洗脱。10 x银羧酸盐表现出上级抗生素和其他银制剂的抗菌活性，并显示出最小的细胞毒性与其他银formulation.Discussion/临床相关性：目前在伤口护理和手术抗菌抗菌治疗，如葡萄糖酸氯己定，有缺陷，包括皮肤渗透性差，疗效持续时间短。这种AgCar制剂的广泛抗微生物活性、延长洗脱和深层皮肤渗透性显示出手术部位感染和伤口护理治疗的巨大前景。对抗日益增长的微生物耐药性威胁的新技术应该处于骨科手术部位感染预防和治疗的最前沿。
Methods:Through an Institutional Animal Care and Use Committee and IRB-approved protocol, AgCar was applied to live Yucatan porcine skin and histologically analyzed for skin penetration. Graphite Furnace Atomic Absorption Spectroscopy (GFAAS) was used to measure elution of AgCar. Dose–response curves were generated through optical density to assess potency. Finally, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay was used to quantify the cellular cytotoxicity of the novel formulation. The results were subject to statistical analysis using analysis of variance and post hoc Tukey tests.Results:The silver carboxylate coating demonstrated deep penetration into the epithelium at the level of the deep pilosebaceous glands in animal models. GFAAS testing demonstrated the extended elution profile of silver carboxylate over 96 hours, while 100% silver with no titanium dioxide-polydimethylsiloxane matrix fully eluted within 48 hours. 10x silver carboxylate demonstrated superior antimicrobial activity to antibiotics and other silver formulations and showed minimal cytotoxicity compared with other silver formulations.Discussion/Clinical Relevance:Current antimicrobial therapies in wound care and surgical antisepsis, such as chlorhexidine gluconate, have pitfalls including poor skin penetration and short duration of efficacy. The broad antimicrobial activity, extended elution, and deep skin penetration of this AgCar formulation show great promise for surgical site infection and wound care treatment. Novel technology to fight the growing threat of microbial resistance should be at the forefront of orthopaedic surgical site infection prevention and treatment.