The tylosin-biosynthetic genes of Streptomyces fradiae
The tylosin-biosynthetic genes of Streptomyces fradiae
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DOI：
10.1023/a:1012065300116
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发表时间：
2001-01-01
影响因子：
2.6
通讯作者：
Merson-Davies, L
中科院分区：
文献类型：
--
作者：
Cundliffe, E;Bate, N;Merson-Davies, L
tyloin -biosynthetic （tyl）基因簇占据了传统链霉菌基因组的1%左右，包括至少43个开放阅读框。除了产生泰洛蛋白所需的结构基因外，泰洛蛋白簇还包含三个抗性决定因素和几个调控基因。泰洛素的产生明显受到途径特异性和多效性调节因子的控制，可能与γ -丁内酯信号因子有关。聚酮苷元的积累由糖基化的大环内酯控制，而复合聚酮合成酶的最佳性能需要编辑硫酯酶的活性。
The tylosin-biosynthetic (tyl) gene cluster occupies about 1% of the genome of Streptomyces fradiae and includes at least 43 open reading frames. In addition to structural genes required for tylosin production, the tyl cluster contains three resistance determinants and several regulatory genes. Tylosin production is evidently controlled by pathway-specific and pleiotropic regulators with the likely involvement of gamma -butyrolactone signalling factors. Accumulation of the polyketide aglycone is controlled by glycosylated macrolides and optimal performance of the complex polyketide synthase enzyme requires the activity of an editing thioesterase.