Protein expression and refolding--a practical guide to getting the most out of inclusion bodies.
Protein expression and refolding--a practical guide to getting the most out of inclusion bodies.
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DOI：
10.1016/s1387-2656(04)10002-1
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Bottomley, Stephen P
中科院分区：
文献类型：
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作者：
Cabrita, Lisa D;Bottomley, Stephen P
The release of sequence data, particularly from a number of medically and biotechnologically important genomes, is increasing in an exponential fashion. In light of this, elucidating the structure and function of proteins, particularly in a "high throughput" manner, is an important quest. The production of recombinant proteins however is not always straightforward, with a number of proteins falling prey to low expression problems, a high susceptibility to proteolysis and the often despised production of inclusion bodies. Whilst expression as inclusion bodies can often be advantageous, their solubilization and renaturation is often a time consuming and empirical process. In this review, we aim to outline some of the more common approaches that have been applied to a variety of proteins and address issues associated with their handling.
