Basal lamina molecules are concentrated in myogenic regions of the mouse limb bud.
Basal lamina molecules are concentrated in myogenic regions of the mouse limb bud.
复制标题
基底层分子集中在小鼠肢芽的生肌区域。
DOI：
10.1007/s004290050198
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Gradall,KS
中科院分区：
文献类型：
--
作者：
Godfrey,EW;Gradall,KS
基底膜的分子成分，如层粘连蛋白，在培养中刺激骨骼肌细胞的分化，而间质基质成分，如纤维连接蛋白，则是抑制的。然而，细胞外基质(ECM)分子在胚胎肌细胞分化中的作用还不是很清楚。作为了解细胞外基质在胚胎肌肉发生中作用的第一步，用免疫荧光法测定了小鼠肢芽中肌细胞分化前和分化过程中基膜分子的定位。在骨骼肌细胞分化前和分化过程中，层粘连蛋白、IV型胶原和Nidogen(Entactin)均集中在肢芽的肌源性区域。与肢体间充质的其他区域相比，基底膜分子的点状免疫荧光主要集中在背侧和腹侧的前肌和肌团。相反，纤维连接蛋白，一种间质细胞外基质分子，在前肌肉和肌肉肿块中的免疫荧光降低。这些结果表明，在发育中的小鼠肢芽中，基板成分在骨骼肌分化的早期阶段起着重要的刺激作用。
Molecular components of basal lamina, such as laminin, stimulate the differentiation of skeletal muscle cells in culture, while interstitial matrix components such as fibronectin are inhibitory. However, the role of extracellular matrix (ECM) molecules in muscle cell differentiation in the embryo is less well understood. As a first step toward understanding the role of the ECM in embryonic myogenesis, the localization of basal lamina molecules in the mouse limb bud before and during muscle cell differentiation was determined by immunofluorescence. Laminin, collagen type IV and nidogen (entactin) were concentrated in myogenic regions of the limb bud both before and during differentiation of skeletal muscle cells. Punctate immunofluorescence for basal lamina molecules was concentrated in dorsal and ventral premuscle and muscle masses, when compared with other regions of limb mesenchyme. In contrast, immunofluorescence for fibronectin, an interstitial extracellular matrix molecule, was decreased in premuscle and muscle masses. These results suggest that basal lamina components play an important stimulatory role in early stages of skeletal muscle differentiation in the developing mouse limb bud.