Protection against influenza virus challenge by topical application of influenza DNA vaccine
Protection against influenza virus challenge by topical application of influenza DNA vaccine
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DOI：
10.1016/s0264-410x(01)00194-3
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发表时间：
2001-08-14
期刊：
影响因子：
5.5
通讯作者：
Okuda, K
中科院分区：
文献类型：
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作者：
Watabe, S;Xin, KQ;Okuda, K
我们研究了表达流感病毒A/PR/8/34的基质（M）基因的DNA疫苗的使用。通过在去除皮肤角质细胞层后将DNA疫苗涂在皮肤上三次来免疫小鼠。用此方法免疫小鼠，产生M特异性抗体和细胞毒性T淋巴细胞（CTL）应答，并获得对流感病毒攻击的抗性。通过体内注射抗CDS或抗CD 4单克隆抗体，这种保护被废除。我们进一步发现，同时局部应用（t.a.）GM-CSF表达质粒（pGM-CSF）或脂质体加甘露聚糖均能增强免疫应答能力，增强对流感病毒攻击的保护作用。本研究表明，通过局部应用给予DNA疫苗可以引起体液和细胞介导的免疫（CMI）。(C)2001爱思唯尔科技有限公司版权所有。
We studied the use of a DNA vaccine expressing the matrix (M) gene of the influenza virus A/PR/8/34. Mice were immunized by painting the DNA vaccine three times on the skin after removal of its keratinocytic layers. Immunization by this method produced M-specific antibodies and cytotoxic T lymphocyte (CTL) response, and acquired resistance against influenza virus challenge. This protection was abrogated by the in vivo injection of anti-CDS or anti-CD4 monoclonal antibody. We further found that simultaneous topical application (t.a.) of GM-CSF expression plasmid (pGM-CSF) or liposomes plus mannan produced stronger immune response competence and enhanced the protective effect against influenza virus challenge. The present study revealed that administering DNA vaccine by topical application can elicit both humoral and cell-mediated immunity (CMI). (C) 2001 Elsevier Science Ltd. All rights reserved.