MOLECULAR CHARACTERIZATION OF CLONED AVIRULENCE GENES FROM RACE-0 AND RACE-1 OF PSEUDOMONAS-SYRINGAE PV GLYCINEA
MOLECULAR CHARACTERIZATION OF CLONED AVIRULENCE GENES FROM RACE-0 AND RACE-1 OF PSEUDOMONAS-SYRINGAE PV GLYCINEA
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DOI：
10.1128/jb.169.12.5789-5794.1987
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发表时间：
1987-12-01
影响因子：
3.2
通讯作者：
NAPOLI, C
中科院分区：
文献类型：
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作者：
STASKAWICZ, B;DAHLBECK, D;NAPOLI, C
构建了寄主范围广的粘粒克隆载体pLAFR3，用于构建丁香假单胞菌小种0号和1号生理小种部分消化的Sau3A DNA粘粒文库。甘西尼娜。从0号生理小种克隆的两个无毒基因avrB0和avrC在特定大豆品种上引发了过敏性反应。含有avrB0的4号小种接合子在24 h内诱导大豆品种Harosoy和NorHead产生深褐色坏死性HR，而含有avrC的4号小种接合子在48 h内诱导Acme、Peking、NorHead和Flambeau品种发生浅褐色坏死性HR。从1号小种克隆的另一个无毒基因avrB1似乎与0号小种的avrB0相同。对0号小种的avrB0和avrC基因进行了限制性内切酶图谱分析。Southern杂交分析、Tn5转座子突变和定点基因替换。还考察了这三个基因对4号生理小种接合子细菌生长的影响。AvrB1的鉴定和克隆为大豆白叶枯病的基因对基因互作提供了遗传证据，因为来自1号小种的avrB1与大豆抗病基因Rpg1相互作用。
A wide-host-range cosmid cloning vector, pLAFR3, was constructed and used to make cosmid libraries of partially digested Sau3A DNA from race 0 and race 1 of Pseudomonas syringae pv. glycinea. Two avirulence genes, avrB0 and avrC, cloned from race 0, elicited the hypersensitivity reaction (HR) on specific cultivars of soybean. Race 4 transconjugants containing avrB0 induced a dark brown necrotic HR within 24 h on the soybean cultivars Harosoy and Norchief, whereas race 4 transconjugants containing avrC induced a light brown necrotic HR within 48 h on the soybean cultivars Acme, Peking, Norchief, and Flambeau. An additional avirulence gene, avrB1, cloned from race 1, appeared to be identical to avrB0 from race 0. The avrB0 and avrC genes from race 0 were characterized by restriction enzyme mapping. Southern blot analysis, Tn5 transposon mutagenesis, and site-directed gene replacements. The effects of these three genes on the in planta bacterial growth of race 4 transconjugants have also been examined. The identification and cloning of avrB1 provides genetic evidence for a gene-for-gene interaction in the bacterial blight disease of soybean, as avrB1 from race 1 interacts with the soybean disease resistance locus, Rpg1.