Evidence for expression of both classical and non-classical major histocompatibility complex class I genes in bovine trophoblast cells
Evidence for expression of both classical and non-classical major histocompatibility complex class I genes in bovine trophoblast cells
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DOI：
10.1111/j.1600-0897.2005.00364.x
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发表时间：
2006-03-01
影响因子：
3.6
通讯作者：
Schlafer, DH
中科院分区：
文献类型：
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作者：
Davies, CJ;Eldridge, JA;Schlafer, DH
问题在妊娠晚期，胎盘间区和胎盘弓区的滋养层细胞表达主要组织相容性复合体I类（MHC-I）抗原。在分娩时，MHC-I抗原的免疫识别似乎有助于正常的胎盘释放。研究方法本研究采用克隆、基因芯片筛选和cDNA序列测定的方法，对妊娠晚期牛外周血单个核细胞（PBMC）和胎盘间滋养层细胞中MHC-I基因的转录进行了研究。结果在MHC-I基因芯片上筛选克隆的MHC-I cDNA，发现PBMC中MHC-I转录本90-98%由经典MHC-I基因编码，其余由非经典MHC-I基因编码。相比之下，胎盘间滋养层细胞的MHC-I转录物的21-66%来自经典基因，34-79%来自非经典基因。通过序列分析鉴定了来自四个非经典MHC-I位点的转录物。结论牛胎盘间质滋养层细胞既表达经典型MHC-Ⅰ类基因，也表达非经典型MHC-Ⅰ类基因，但相对表达量差异较大。
Problem During the third trimester of pregnancy bovine trophoblast cells in the interplacentomal and arcade regions of the placenta express major histocompatibility complex class I (MHC-I) antigens. At parturition immunological recognition of MHC-I antigens appears to contribute to normal placental release. Therefore, we hypothesized that during late pregnancy bovine trophoblast cells express polymorphic, classical MHC-I antigens.Method of study Cloning, microarray screening and sequencing of cDNA were used to study transcription of MHC-I genes in peripheral blood mononuclear cells (PBMC) and interplacentomal, trophoblast cells. Real-time reverse transcription, polymerase chain reaction (RT-PCR) was used to compare the abundance of MHC-I transcripts in PBMC and trophoblast cells.Results Screening of cloned MHC-I cDNA on MHC-I microarrays indicated that in PBMC 90-98% of MHC-I transcripts were encoded by classical MHC-I genes with the remainder encoded by non-classical MHC-I genes. In contrast, 21-66% of MHC-I transcripts from interplacentomal trophoblast cells were from classical genes and 34-79% were from non-classical genes. Transcripts from four non-classical MHC-I loci were identified by sequence analysis. Real-time RT-PCR indicated that the overall levels of MHC-I gene expression in PBMC and trophoblast were similar.Conclusion Bovine interplacentomal trophoblast cells express both classical and non-classical MHC-I genes, but the relative level of expression varies considerably.