Mantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cells.
Mantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cells.
复制标题
Mantis：细胞分子和物理结构的高通量 4D 成像和分析。
DOI：
10.1101/2023.12.19.572435
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Mehta,ShalinB
中科院分区：
文献类型：
--
作者：
Ivanov,IvanE;Hirata-Miyasaki,Eduardo;Chandler,Talon;Kovilakam,RasmiCheloor;Liu,Ziwen;Liu,Chad;Leonetti,ManuelD;Huang,Bo;Mehta,ShalinB
细胞和细胞器的高通量动态成像对于理解复杂的细胞反应是必不可少的。我们报告了Mantis，这是一种高通量4D显微镜，它集成了两种互补的、温和的活细胞成像技术：远程重新聚焦无标记显微镜和倾斜光片荧光显微镜。此外，我们报告了ShrimPy(智能高通量稳健成像和测量)，这是一个开源软件，用于4D数据的高通量成像、去卷积和单细胞表型鉴定。使用Mantis和Shrimpy，我们实现了在7.5h内每15分钟对20个细胞系的分子动力学和物理结构的高含量相关成像。该平台还有助于详细测量病毒感染对宿主细胞和宿主蛋白结构的影响。Mantis平台可以实现对细胞内动力学的高通量分析、对细胞对扰动的反应的长期成像和分析，以及剖析基因调控网络的活细胞光学屏幕。
High-throughput dynamic imaging of cells and organelles is essential for understanding complex cellular responses. We report Mantis, a high-throughput 4D microscope that integrates two complementary, gentle, live-cell imaging technologies: remote-refocus label-free microscopy and oblique light-sheet fluorescence microscopy. Additionally, we report shrimPy (Smart High-throughput Robust Imaging and Measurement in Python), an open-source software for high-throughput imaging, deconvolution, and single-cell phenotyping of 4D data. Using Mantis and shrimPy, we achieved high-content correlative imaging of molecular dynamics and the physical architecture of 20 cell lines every 15 min over 7.5 h. This platform also facilitated detailed measurements of the impacts of viral infection on the architecture of host cells and host proteins. The Mantis platform can enable high-throughput profiling of intracellular dynamics, long-term imaging and analysis of cellular responses to perturbations, and live-cell optical screens to dissect gene regulatory networks.