Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model.
Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model.
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DOI：
10.1038/srep14424
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发表时间：
2015-09-30
影响因子：
4.6
通讯作者：
Shibata S
中科院分区：
文献类型：
--
作者：
Tanabe K;Kitagawa E;Wada M;Haraguchi A;Orihara K;Tahara Y;Nakao A;Shibata S
哺乳动物的生物钟控制着许多生理过程，包括免疫反应和过敏反应。一些研究已经调查了肠道通透性和紧密连接的昼夜调节，已知受细胞因子的影响。然而，生物钟对食物过敏症状的影响尚不清楚。因此，我们研究了生物钟在决定食物过敏严重程度中的作用。我们制备了一个卵清蛋白食物过敏小鼠模型，并在明暗循环下，在光照后期或黑暗后期口服卵清蛋白。光照期组过敏性腹泻和体重下降明显高于暗光照期组。光照组肠系膜淋巴结2型细胞因子IL-13、IL-5的产生及卵清蛋白的吸收均高于暗光照组。与暗期组相比，光期组紧密连接蛋白mRNA表达水平较低。我们已经证明，在轻度时期，2型细胞因子和肠道通透性的增加会引起严重的食物过敏症状。我们的结果提示，食物抗原的摄入时间可能会影响食物过敏症状严重程度的测定。
The mammalian circadian clock controls many physiological processes that include immune responses and allergic reactions. Several studies have investigated the circadian regulation of intestinal permeability and tight junctions known to be affected by cytokines. However, the contribution of circadian clock to food allergy symptoms remains unclear. Therefore, we investigated the role of the circadian clock in determining the severity of food allergies. We prepared an ovalbumin food allergy mouse model, and orally administered ovalbumin either late in the light or late in the dark period under light-dark cycle. The light period group showed higher allergic diarrhea and weight loss than the dark period group. The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group. Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group. We have demonstrated that increased production of type 2 cytokines and intestinal permeability in the light period induced severe food allergy symptoms. Our results suggest that the time of food antigen intake might affect the determination of the severity of food allergy symptoms.