Activation of small heat shock protein (SlHSP17.7) gene by cell wall invertase inhibitor (SlCIF1) gene involved in sugar metabolism in tomato.
Activation of small heat shock protein (SlHSP17.7) gene by cell wall invertase inhibitor (SlCIF1) gene involved in sugar metabolism in tomato.
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DOI：
10.1016/j.gene.2018.08.077
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发表时间：
2018-12
期刊：
影响因子：
3.5
通讯作者：
Ning Zhang;Jiewei Shi;Huaiyin Zhao;Jing Jiang
中科院分区：
文献类型：
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作者：
Ning Zhang;Jiewei Shi;Huaiyin Zhao;Jing Jiang
果实品质的形成涉及果实成熟过程中的一系列生理生化变化。蔗糖代谢不仅在果实成熟过程中起着建立能量状态和营养品质的重要作用，而且在基因表达中也起着非营养作用。在碳代谢和果实成熟过程中，细胞壁转化酶(CWINS)发挥着重要的调节功能。在果实特异性启动子的作用下，β-果糖苷酶的细胞壁抑制因子(CIF)过表达，从而抑制了果实中CWIN的活性，目前对这种基因表达变化的认识还很有限。为了进一步探讨蔗糖调控的分子机制，利用基因芯片技术对转细胞壁转化酶抑制因子基因(SlCIF1)的转基因番茄果实进行了整体表达谱分析。总共鉴定出622个和833个差异表达基因(Deg)。在转SlCIF1番茄中，SLHSP17.7基因的表达增加了几千倍。在此基础上，将pB7GWIWG2(I)-SlHSP17.7导入野生型小麦，获得了SLHSP17.7-RNA干扰(RNAi)系。RNAi处理的果实中蔗糖和果糖含量显著低于对照。此外，沉默SLHSP17.7基因还协同下调了14个糖代谢相关基因。我们的数据表明，SLCIF1对CWIN活性的翻译后调控有助于提早开花。SLHSP17.7和糖可以相互作用，调节番茄果实的发育，影响番茄的品质，为提高番茄的品质提供了不同的视角。
Fruit quality formation involves a series of physiological and biochemical changes during fruit ripening. Sucrose metabolism plays not only important roles in fruit ripening to establish energy status and nutritional quality but also a non-nutritive role in gene expression. In carbon metabolism and fruit ripening, cell wall invertases (CWINs) perform essential regulatory functions. Knowledge regarding the gene expression changes that occur following the repression of CWIN activity in fruit through the overexpression of a cell-wall inhibitor of β-fructosidase (CIF) under a fruit-specific promoter is limited. To further explore the molecular mechanism of sucrose regulation, global expression profiling of the fruits of transgenic tomato (Solanumlycopersicum) plants carrying a cell wall invertase inhibitor (SlCIF1) gene was performed using a microarray. In total, 622 and 833 differentially expressed genes (DEGs) were identified. The expression of theSlHSP17.7gene was increased by thousands of times in the transgenic-SlCIF1tomato. Then, SlHSP17.7-RNA interference (RNAi) lines were generated by introducing pB7GWIWG2 (I)-SlHSP17.7 into wild-typechmielewskiitomatoes (WT). The sucrose and fructose contents significantly decreased in the RNAi fruits compared with those in the WT. Furthermore, 14 sugar metabolism related genes were also decreased synergistically by silencingSlHSP17.7gene. Our data indicate that the posttranslational modulation of CWIN activity bySlCIF1contributes to earlier bloom times.SlHSP17.7and sugar can interact to regulate the development of tomato fruit and affect the quality of tomato, providing a different insight into improving the quality of tomato.