Comparison of two flow-based imaging methods to measure individual red blood cell area and volume
Comparison of two flow-based imaging methods to measure individual red blood cell area and volume
复制标题
DOI：
10.1002/cyto.a.22215
复制
发表时间：
2012-12-01
期刊：
影响因子：
3.7
通讯作者：
Guido, Stefano
中科院分区：
文献类型：
--
作者：
Tomaiuolo, Giovanna;Rossi, Domenico;Guido, Stefano
红细胞（RBC）群体的特征在于膜面积、细胞体积和机械性质的高度异质性，这主要是由于红细胞在其整个寿命期间经历的各种机械和化学应力。在这里，我们提供了第一个同时的面积和体积测量的红细胞在微毛细管中流动，通过使用高速视频显微镜成像和定量数据处理的基础上的图像分析技术。受限和无界流动条件（取决于红细胞的相对大小和微毛细管直径）进行了研究。将结果与文献中的微量移液器实验和Coulter计数器常规临床血液检测数据进行比较。发现RBC体积具有良好的一致性，特别是在受限流条件下。常规临床试验中缺乏的表面积测量作为改变的细胞变形性和聚集性的潜在诊断参数特别令人感兴趣。总的来说，我们的研究结果提供了一种新的流动方法，适用于高通量测量RBC的几何参数，使人们能够克服经典的静态方法，如微量吸液管抽吸，这是不适合处理大量的细胞的限制。(C)2012年国际细胞计数促进学会
The red blood cells (RBCs) population is characterized by a high heterogeneity in membrane area, cellular volume, and mechanical properties, mainly due to the variety of mechanical and chemical stresses that a red cell undergoes in its entire life span. Here, we provide the first simultaneous area and volume measurements of RBCs flowing in microcapillaries, by using high-speed video microscopy imaging and quantitative data processing based on image analysis techniques. Both confined and unbounded flow conditions (depending on the relative size of RBCs and microcapillary diameter) are investigated. The results are compared with micropipette experiments from the literature and data from Coulter counter routine clinical blood tests. Good agreement is found for RBC volume, especially in the case of confined flow conditions. Surface area measurements, which are lacking in the routine clinical test, are of special interest being a potential diagnostic parameter of altered cell deformability and aggregability. Overall, our results provide a novel flow methodology suitable for high-throughput measurements of RBC geometrical parameters, allowing one to overcome the limits of classical static methods, such as micropipette aspiration, which are not suitable for handling a large number of cells. (C) 2012 International Society for Advancement of Cytometry