Mechanism of action of the novel herbicide ZJ0273
Mechanism of action of the novel herbicide ZJ0273
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发表时间：
2005
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通讯作者：
Yuan Jun
中科院分区：
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作者：
Yuan Jun
采用常规生物测定和生化方法研究了新型除草剂ZJ 0273的作用机理，结果表明，在ZJ 0273处理的高粱幼苗培养物中添加5 mg/L的3种支链氨基酸完全缓解了除草剂诱导的茎生长抑制，而添加的瓦尔、在相同浓度下，Ile和Leu单用对ZJ 0273诱导的生长抑制仅有部分逆转作用，ZJ 0273和双草醚的IC_（50）值分别大于100 mg/L和10~（-5）~ 100 mg/L。ZJ 0273对乙酰乳酸合成酶（ALS）的抑制作用不明显，但对ALS的体内抑制作用显著，且随着处理时间的延长，ALS的活性逐渐降低，说明ZJ 0273抑制了必需分支氨基酸的生物合成，具有ALS抑制剂的作用。ZJ 0273的作用方式与其他典型的市售ALS抑制剂（如磺酰脲类和嘧啶水杨酸类除草剂）不同，其作用机制与前除草剂相似，由植物体内的代谢过程激活。
The mechanism of the novel herbicide ZJ0273 is studied with the conventional bioassay and bio- chemical methods.The results indicated that the addition of 5 mg/L of 3 branched-amino acids to sorghum seedling cultures incubated in the presence of ZJ0273 completely alleviated herbicide-induced stem growth in- hibition.But each of Val,Ile and Leu single was only partly effective in reversing ZJ0273-induced growth in- hibition at the same concentrations.The IC_(50) values of ZJ0273 and bispyribac-sodium were respectively over 100 mg/L and in the ranges of 10~(-5)-10~(-4)mg/L in vitro,so ZJ0273 showed little inhibition effect on acetolac- rate synthase (ALS).ALS was greatly inhibited by ZJ0273 in vivo,and the activity of ALS was lowered when the treat time increasing.It is concluded that ZJ0273 inhibited biosynthesis of the essential branch amino acids and acted as an ALS inhibitor.However,the mode of action of ZJ0273 differs from the other typically commer- cial ALS inhibitors,such as sulfonylurea and pyrimidine salicylic acid herbicides.Its mechanism is similar to a pre-herbicide,and activated by the metabolic processes in the plants.