Protein glycosylation in Parelaphostrongylus tenuis -: first description of the Galα1-3Gal sequence in a nematode
Protein glycosylation in Parelaphostrongylus tenuis -: first description of the Galα1-3Gal sequence in a nematode
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DOI：
10.1093/glycob/cwl001
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发表时间：
2006-09-01
期刊：
影响因子：
4.3
通讯作者：
Haslam, Stuart M.
中科院分区：
文献类型：
--
作者：
Duffy, Michael S.;Morris, Howard R.;Haslam, Stuart M.
白尾鹿是细圆圆线虫寄生线虫的最终宿主。这种寄生虫还会感染多种家畜，导致衰弱的神经系统疾病。糖缀合物越来越多地参与线虫策略，以维持免疫能力强的宿主的持续感染。在这项研究中，我们结合经典生化技术（包括蛋白质印迹和抗碳水化合物单克隆抗体免疫组织化学染色）进行了详细的质谱分析，并表明 P. tenuis 含有复合型 N-聚糖，触角覆盖有 Gal α 1-3Gal beta 1-4GlcNAc 序列。通过模仿脊椎动物聚糖，Gal α 1-3Gal 可以帮助寄生虫逃避宿主的免疫检测。这是线虫中 Gal α 1-3Gal 序列的首次报道。
The white-tailed deer is the definitive host of the parasitic nematode Parelaphostrongylus tenuis. This parasite also infects a wide variety of domesticated livestock, causing a debilitating neurologic disease. Glycoconjugates are becoming increasingly implicated in nematode strategies to maintain persistent infections in immunologically competent hosts. In this study, we have carried out detailed mass spectrometric analysis together with classical biochemical techniques, including western blotting and inummohistochemical staining with anticarbohydrate monoclonal antibodies and have shown that P. tenuis contains complex-type N-glycans with the antennae capped with Gal alpha 1-3Gal beta 1-4GIcNAc sequence. By mimicking a vertebrate glycan, Gal alpha 1-3Gal may aid the parasite in evading immunological detection by the host. This is the first report of the Gal alpha 1-3Gal sequence in a nematode.