Analysis of Drug-Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin
Analysis of Drug-Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin
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DOI：
10.1007/978-1-4939-2447-9_21
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Hage, David S.
中科院分区：
文献类型：
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作者：
Matsuda, Ryan;Anguizola, Jeanethe;Hage, David S.
高效亲和色谱（HPAC）是一种液相色谱，已被越来越多地用作研究药物-蛋白质相互作用的工具。本报告介绍了如何HPAC可以用来提供信息的结合位点的数量，平衡常数，并在结合过程中可能发生的药物-蛋白质相互作用的变化。这种方法将通过HPAC获得的磺酰脲类药物和其他溶质与蛋白质人血清白蛋白（HSA）结合的最新数据来说明，特别是这种蛋白质的非酶糖化修饰形式。在这项工作中，前沿分析和分区洗脱竞争研究的理论和使用将被讨论。将介绍这些实验的各个实际方面，以及将这些方法扩展到其他药物和蛋白质或其他类型的生物相互作用时要考虑的因素。
High-performance affinity chromatography (HPAC) is a type of liquid chromatography that has seen growing use as a tool for the study of drug-protein interactions. This report describes how HPAC can be used to provide information on the number of binding sites, equilibrium constants, and changes in binding that can occur during drug-protein interactions. This approach will be illustrated through recent data that have been obtained by HPAC for the binding of sulfonylurea drugs and other solutes to the protein human serum albumin (HSA), and especially to forms of this protein that have been modified by non-enzymatic glycation. The theory and use of both frontal analysis and zonal elution competition studies in such work will be discussed. Various practical aspects of these experiments will be presented, as well as factors to consider in the extension of these methods to other drugs and proteins or additional types of biological interactions.