Antimicrobial activity of Trembleya laniflora , Xyris platystachia and Xyris pterygoblephara

Antimicrobial activity of Trembleya laniflora , Xyris platystachia and Xyris pterygoblephara
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Trembleya laniflora 、 Xyris platystachia 和 Xyris pterygoblephara 的抗菌活性

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发表时间:
2007
期刊:
影响因子:
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通讯作者:
F. Braga
F. Braga
中科院分区:
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文献类型:
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作者:
C. P. Ventura;A. Oliveira;F. Braga

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Trembleya laniflora(D.唐)科恩。(木贼科)、宽叶木舟蛾Xyris platystachia Alb. Nilss.(Xyridaceae)和翼珠木Xyris pterygoblephara Kunth.,从生物多样性热点地区收集的巴西物种优先保护,其抗菌活性对标准菌株的金黄色葡萄球菌和微球菌黄,琼脂扩散试验进行了评价。在2000 µg/片浓度下测定的所有提取物均具有抗M活性。luteus,而S.金黄色葡萄球菌生长仅被T. laniflora叶和X.平果类地上部分。通过在不混溶的溶剂之间进行分配而对提取物进行分级,从最初对S.金黄色。活性主要存在于低极性和中极性馏分中。T.通过硅胶柱层析对羊毛花叶进行了分离,所得组分的HPLC指纹图谱和抗菌活性均与来自分配过程的组分不同。
Trembleya laniflora (D. Don) Cogn. (Melastomataceae), Xyris platystachia Alb. Nilss. (Xyridaceae) and Xyris pterygoblephara Kunth., Brazilian species collected from a biodiversity hotspot for conservation priority, had their antimicrobial activity evaluated against standardized strains of Staphylococcus aureus and Micrococcus luteus, by the agar diffusion assay. All extracts, assayed in the concentration of 2000 µg/disc, were active against M. luteus, whereas S. aureus growth was inhibited only by T. laniflora leaves and X. platystachia aerial parts. Fractionation of the extracts by partition between immiscible solvents resulted in active fractions from extracts originally inactive against S. aureus. Activity was mainly found in low and medium polar fractions. The extract of T. laniflora leaves was also fractionated by silica gel column chromatography and both the HPLC fingerprint and antimicrobial activity of the obtained fractions were distinct of those originated from the partition process.