DNA methylation variation in cloned mice
DNA methylation variation in cloned mice
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DOI：
10.1002/gene.1031
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发表时间：
2001-06-01
期刊：
影响因子：
1.5
通讯作者：
Shiota, K
中科院分区：
文献类型：
--
作者：
Ohgane, J;Wakayama, T;Shiota, K
哺乳动物克隆已经在几种哺乳动物物种中通过核转移完成。然而，克隆动物的生产率很低，许多克隆后代死亡或出现异常症状，克隆成功率低和许多克隆动物出现异常症状的一个可能原因是核移植后DNA甲基化重建不完全。在检查的三种不同组织中，有七个点/CpG岛（占检测到的总CpG岛的0.5%）不同地甲基化。在两个克隆胎儿的胎盘和皮肤中，共有4个CpG岛发生了异常甲基化或未甲基化，有趣的是，这4个基因座中的3个与正常对照胎儿的组织特异性基因座相对应。不同的克隆动物存在不同的甲基化畸变，也就是说，克隆动物的基因组DNA甲基化状态并不是原始动物的完美拷贝。(C)2001 Wiley-Liss，Inc.
Mammalian cloning has been accomplished in several mammalian species by nuclear transfer. However, the production rate of cloned animals is quite low, and many cloned offspring die or show abnormal symptoms, A possible cause of the low success rate of cloning and abnormal symptoms in many cloned animals is the incomplete reestablishment of DNA methylation after nuclear transfer, We first analyzed tissue-specific methylation patterns in the placenta, skin, and kidney of normal B6D2F1 mice. There were seven spots/CpG islands (0.5% of the total CpG islands detected) methylated differently in the three different tissues examined. In the placenta and skin of two cloned fetuses, a total of four CpG islands were aberrantly methylated or unmethylated, Interestingly, three of these four loci corresponded to the tissue-specific loci in the normal control fetuses, The extent of aberrant methylation of genomic DNA varied between the cloned animals, In cloned animals, aberrant methylation occurred mainly at tissue-specific methylated loci. Individual cloned animals have different methylation aberrations, In other words, cloned animals are by no means perfect copies of the original animals as far as the methylation status of genomic DNA is concerned. (C) 2001 Wiley-Liss, Inc.