Impaired insulin action in subcutaneous adipocytes from women with visceral obesity
Impaired insulin action in subcutaneous adipocytes from women with visceral obesity
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DOI：
10.1152/ajpendo.2001.280.1.e40
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发表时间：
2001-01-01
影响因子：
5.1
通讯作者：
Albu, JB
中科院分区：
文献类型：
--
作者：
Johnson, JA;Fried, SK;Albu, JB
内脏肥胖与体内胰岛素抗脂解作用的抵抗有关。我们研究了内脏肥胖女性的皮下腹部和臀部脂肪细胞是否在体外表现出胰岛素抵抗。受试者是肥胖的黑人和白人绝经前非糖尿病女性，其内脏脂肪组织（VAT）、总肥胖量和年龄相匹配。与种族和脂肪细胞大小无关，VAT 增加与皮下腹部和臀肌脂肪细胞对胰岛素抗脂解作用的敏感性降低相关。在生理相关浓度的胰岛素或腺苷受体激动剂 N-6-（苯基异丙基）腺苷下，VAT 面积最高的受试者与最低的受试者相比，绝对脂肪分解率较高。与细胞大小无关，腹部脂肪细胞对胰岛素的抗脂解作用的敏感性低于臀肌脂肪细胞，这可能部分解释了上半身肥胖女性与下半身肥胖女性的非酯化脂肪酸通量增加。此外，VAT 增加与胰岛素对腹部脂肪细胞葡萄糖转运的刺激作用的反应性降低有关，但与敏感性降低无关。这些数据表明，腹部皮下脂肪细胞以及臀部脂肪细胞的胰岛素抵抗（在较小程度上）可能导致黑人和白人内脏肥胖女性的全身脂肪分解增加。
Visceral obesity is associated with resistance to the antilipolytic effect of insulin in vivo. We investigated whether subcutaneous abdominal and gluteal adipocytes from viscerally obese women exhibit insulin resistance in vitro. Subjects were obese black and white premenopausal nondiabetic women matched for visceral adipose tissue (VAT), total adiposity, and age. Independently of race and adipocyte size, increased VAT was associated with decreased sensitivity to insulin's antilipolytic effect in subcutaneous abdominal and gluteal adipocytes. Absolute lipolytic rates at physiologically relevant concentrations of insulin or the adenosine receptor agonist N-6-( phenylisopropyl) adenosine were higher in subjects with the highest vs. lowest VAT area. Independently of cell size, abdominal adipocytes were less sensitive to the antilipolytic effect of insulin than gluteal adipocytes, which may partly explain increased nonesterified fatty acid fluxes in upper vs. lower body obese women. Moreover, increased VAT was associated with decreased responsiveness, but not decreased sensitivity, to insulin's stimulatory effect on glucose transport in abdominal adipocytes. These data suggest that insulin resistance of subcutaneous abdominal and, to a lesser extent, gluteal adipocytes may contribute to increased systemic lipolysis in both black and white viscerally obese women.