Carbon sources mediate microbial pentachlorophenol dechlorination in soils.
Carbon sources mediate microbial pentachlorophenol dechlorination in soils.
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DOI：
10.1016/j.jhazmat.2019.03.109
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发表时间：
2019-07
影响因子：
13.6
通讯作者：
Hui Li;Yuji Jiang;Lijun Chen;Yating Chen;Xiaocui Wen;Liang Tao
中科院分区：
文献类型：
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作者：
Hui Li;Yuji Jiang;Lijun Chen;Yating Chen;Xiaocui Wen;Liang Tao
本研究采用网络分析的方法，研究了空白、柠檬酸、葡萄糖和乳酸等碳源对两种土壤中原生细菌群落和五氯苯酚(PCP)脱氯的影响。动力学结果表明，添加柠檬酸盐/乳酸可显著提高PCP的脱氯率，但添加葡萄糖对PCP的脱氯率影响较小。高通量测序结果表明，在PCP的脱氯过程中，4种不同处理的优势菌群均为细菌和变形杆菌，而随机森林分析表明，梭状芽胞杆菌、盐质芽孢杆菌、芽孢杆菌、假单胞菌和盖氏杆菌是与PCP脱氯显著相关的模块中的关键细菌。其中，在柠檬酸和乳酸处理下，梭菌的相对丰度均显著增加，进一步加速了PCP的脱氯。添加柠檬酸/乳酸作为碳源，提高了细菌共生网络密度、平均聚集度和模块化程度。此外，与葡萄糖/空白网络相比，柠檬酸/乳酸网络中与PCP脱氯显著相关的模块更多。随机森林模型表明，梭形藻类在这些功能模块中发挥了关键作用。综上所述，我们的研究结果揭示了外源碳源通过改变土壤细菌网络影响PCP脱氯途径的生物学机制。
In this study, experiments were performed using network analysis to investigate the effects of different carbon sources, including blank, citrate, glucose and lactate, on indigenous bacterial communities and on the pentachlorophenol (PCP) dechlorination in two soils. Kinetics results demonstrate that PCP dechlorination is significantly enhanced by adding citrate/lactate, but to a lesser extent by adding glucose. High-throughput sequencing results revealed thatFirmicutesandProteobacteriawere the dominant groups in these four different treatments during the PCP dechlorination, whereas random forest analysis indicated that the ordersClostridiales, Haloplasmatales, Bacillales, PseudomonadalesandGaiellaleswere the critical bacterial orders in modules that were significantly correlated with PCP dechlorination. Among them, the relative abundance ofClostridialesdramatically increased in both citrate and lactate treatment, further accelerating the PCP dechlorination. Addition of citrate/lactate as the carbon source increased the bacterial co-occurrence network density, average clustering coefficient and modularity. Moreover, more modules significantly correlated with PCP dechlorination in the citrate/lactate networks compared with the glucose/blank networks. Random forest modeling suggested thatClostridialesplayed a critical role in these functional modules. Taken together, our results provide insight into the biological mechanism of the impact of exogenous carbon sources on PCP dechlorination pathways by modifying soil bacterial networks.