PYOCHELIN - NOVEL STRUCTURE OF AN IRON-CHELATING GROWTH PROMOTER FOR PSEUDOMONAS-AERUGINOSA
PYOCHELIN - NOVEL STRUCTURE OF AN IRON-CHELATING GROWTH PROMOTER FOR PSEUDOMONAS-AERUGINOSA
复制标题
DOI：
10.1073/pnas.78.7.4256
复制
发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
COOK, JC
中科院分区：
文献类型：
--
作者：
COX, CD;RINEHART, KL;COOK, JC
从包括临床菌株在内的铜绿假单胞菌中分离到一种内源性生长促进剂，它能溶解铁。褐球蛋白的结构是2-(2-o-hydroxyphenyl-2-thiazolin-4-yl)-3-methylthiazolidine-4-carboxylic酸，与以前从细菌中分配给铁载体(铁铬)的结构不同。这项任务依赖于1H和13C核磁共振数据、高分辨率(包括场解吸)质谱学和合成模型化合物的光谱性质。该蛋白被认为是由水杨酸和2摩尔的半胱氨酸生物合成的。
Pyochelin, an endogenous growth promoter that solubilizes ferric iron, was isolated from P. aeruginosa, including clinical strains. The structure of pyochelin was 2-(2-o-hydroxyphenyl-2-thiazolin-4-yl)-3-methylthiazolidine-4-carboxylic acid and is of a different type from those previously assigned to siderophores (siderochromes) from bacteria. The assignment rests on 1H and 13C NMR data, high-resolution (including field desorption) mass spectrometry, and spectroscopic properties of synthetic model compounds. Pyochelin is presumed to be biosynthesized from salicyclic acid and 2 mol of cysteine.