Long-range Ca2+ signaling from growth cone to soma mediates reversal of neuronal migration induced by Slit-2
Long-range Ca2+ signaling from growth cone to soma mediates reversal of neuronal migration induced by Slit-2
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DOI：
10.1016/j.cell.2007.01.051
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发表时间：
2007-04-20
期刊：
影响因子：
64.5
通讯作者：
Poo, Mu-ming
中科院分区：
文献类型：
--
作者：
Guan, Chen-bing;Xu, Hua-tai;Poo, Mu-ming
神经元迁移和生长锥延伸都是由发育中的大脑中的细胞外因子引导的，但这两种形式的引导是否在机械上相关联尚不清楚。应用Slit-2梯度在前面的领先过程中培养的小脑颗粒细胞导致崩溃的生长锥和逆转的神经元迁移，一个事件之前的传播Ca 2+波从生长锥的索马。Ca 2+波是Slit-2效应所必需的，其本身足以诱导迁移的逆转。Slit-2诱导的迁移逆转需要活跃的RhoA，其在迁移神经元的前部积累，并且这种极化的RhoA分布在由Slit-2梯度或Ca（2+）波诱导的迁移逆转期间被逆转。因此，长程Ca（2+）信号通过调节RhoA的分布，协调了Slit-2诱导的迁移神经元两个远端运动的变化。
Neuronal migration and growth-cone extension are both guided by extracellular factors in the developing brain, but whether these two forms of guidance are mechanistically linked is unclear. Application of a Slit-2 gradient in front of the leading process of cultured cerebellar granule cells led to the collapse of the growth cone and the reversal of neuronal migration, an event preceded by a propagating Ca2+ wave from the growth cone to the soma. The Ca2+ wave was required for the Slit-2 effect and was sufficient by itself to induce the reversal of migration. The Slit-2-induced reversal of migration required active RhoA, which was accumulated at the front of the migrating neuron, and this polarized RhoA distribution was reversed during the migration reversal induced by either the Slit-2 gradient or the Ca (2+) wave. Thus, longrange Ca (2+) signaling coordinates the Slit-2induced changes in motility at two distant parts of migrating neurons by regulating RhoA distribution.