Comparative Genome Analysis of Fish and Human Isolates of Mycobacterium marinum
Comparative Genome Analysis of Fish and Human Isolates of Mycobacterium marinum
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DOI：
10.1007/s10126-013-9511-6
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发表时间：
2013-10-01
影响因子：
3
通讯作者：
Aoki, Takashi
中科院分区：
文献类型：
--
作者：
Kurokawa, Satoru;Kabayama, Jun;Aoki, Takashi
海洋分枝杆菌是鱼类分枝杆菌病的主要病原体，具有广泛的宿主，包括人类分离株。到目前为止，基因组分析主要集中在人类分离株上。在这里，我们比较了两株M. marinum分离自鱼类（MB 2和欧洲）的M. marinum M分离自人类。M. marinumMB 2和Europe的染色体均为单环状，长度分别为6，134，389和6，029，340 bp，平均G + C含量分别为65.7%和65.5%。在两个M. marinum MB 2和欧洲基因组。点图分析表明，M. marinum MB 2和欧洲与M. marinum M与其他三种分枝杆菌相比。插入/缺失基因分析表明，M. marinum MB 2和Europe分别含有M. marinum M中缺失625和776个基因。marinum M。大部分插入和缺失的基因被归类在脂肪酸，脂质和类异戊二烯子系统和毒性，疾病和防御子系统。因此，这些结果提供了与可变宿主和病原体相关的基因组多样性的见解。
Mycobacterium marinum is a major causative agent of mycobacteriosis in fish that has a broad range of hosts, including in human isolates. So far, genomic analyses have focused on the human isolate. Here, we compared the draft genome sequences of two strains of M. marinum isolated from fish (MB2 and Europe) with the M. marinum M isolated from humans. M. marinum MB2 and Europe have single, circular chromosomes of 6,134,389 and 6,029,340 bp, and average G + C contents of 65.7 and 65.5 %, respectively. A total of 5,464 coding DNA sequences were annotated in both M. marinum MB2 and Europe genome. Dot plot analyses showed that M. marinum MB2 and Europe were closer to M. marinum M when compared to three other Mycobacterium species. The insertion/deletion gene analysis showed that M. marinum MB2 and Europe contained 342 and 487 genes that were not found in M. marinum M, and lacked 625 and 776 genes found in M. marinum M, respectively. Most of the inserted and deleted genes were classified in the fatty acid, lipid, and isoprenoid subsystem and the virulence, disease, and defense subsystem. Therefore, these results provide insights into the genomic diversity associated with variable hosts and pathogens.