Allelopathic Influence of Cyanobacteria Microcystis aeruginosa on Green Algae Chlorella vulgaris
Allelopathic Influence of Cyanobacteria Microcystis aeruginosa on Green Algae Chlorella vulgaris
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DOI：
10.1007/978-3-319-03683-0_10
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
A. Zak;A. Kosakowska
中科院分区：
文献类型：
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作者：
A. Zak;A. Kosakowska
不直接参与生物体正常生长、发育和繁殖的有机化合物称为次生代谢物。生物和农业系统的生长和发育可能受到具有生物活性的次生代谢物的影响。这种现象称为化感作用。化感物质可以影响靶生物细胞的不同生理过程，从而导致生长抑制甚至死亡。这项工作的目的是研究蓝藻铜绿微囊藻对绿藻普通小球藻的化感影响。这两个物种都出现在波罗的海；此外，M.铜绿假单胞菌可能在沿海地区局部形成有毒水华（HAB）。在烧瓶中的液体培养基上培养无菌蓝藻单一培养物。生长 30 天后，通过在无菌条件下通过玻璃纤维过滤器过滤来收集来自蓝藻培养物的无细胞溶液，并将其添加到小球藻培养物中。 3 天和 7 天后研究了获得的细胞外分泌物对绿色微藻生长的影响。在其他实验中，研究了活蓝藻细胞对绿藻生长的影响（共培养）。在这两个实验中M。铜绿素对绿藻具有化感作用。
Organic compounds not directly involved in the normal growth, development and reproduction of organism are called secondary metabolites. The growth and development of biological and agricultural systems could be affected by secondary metabolites with biological activity. This phenomenon is known as allelopathy. Allelochemicals could have influence on different physiological processes of target organism cells, which could lead to growth inhibition or even death. The goal of this work was to investigate allelopathic influence of cyanobacteriaMicrocystis aeruginosaon green algaeChlorella vulgaris. Both species occur in Baltic Sea; furthermore,M. aeruginosacould locally form toxic blooms (HABs) in coastal zone. Axenic cyanobacterial monocultures were cultivated in the flasks on liquid medium. After 30 days of growth cell-free solutions from cyanobacterial cultures were collected by filtering through the glass-fiber filters in sterile conditions and added toChlorella vulgariscultures. The influence of obtained extracellular secretion on growth of green microalgae was investigated after 3 and 7 days. In other experiment the influence of living cyanobacterial cells on growth of green algae was investigated (cocultures). In both experimentsM. aeruginosademonstrated allelopathic effects towards green algae.