The RNA Editing Factor SlORRM4 Is Required for Normal Fruit Ripening in Tomato
The RNA Editing Factor SlORRM4 Is Required for Normal Fruit Ripening in Tomato
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RNA编辑因子SlORRM4是番茄果实正常成熟所必需的
DOI：
10.1104/pp.17.01265
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发表时间：
2017
期刊：
影响因子：
7.4
通讯作者：
Zhu Hongliang
中科院分区：
文献类型：
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作者：
Yang Yongfang;Zhu Guoning;Li Rui;Yan Shijie;Fu Daqi;Zhu Benzhong;Tian Huiqin;Luo Yunbo;Zhu Hongliang
RNA编辑在基因表达中起着关键的转录后作用。现有的植物胞苷到尿苷RNA编辑的研究主要集中在玉米(Zea Mays)、水稻(Oryza Sativa)和拟南芥(Arabitopsis Thaliana)。然而，RNA编辑在几个关键的农艺过程中的重要性和调节还没有被很好地理解，其中一个显著的例子是果实成熟。在这里，我们分析了33个RNA编辑因子的表达谱，并确定了11个可能与番茄(Solanum Lycopsicum)果实成熟相关的因子。病毒诱导的快速基因沉默实验表明，细胞器RNA识别基序蛋白SlORRM4影响番茄果实成熟。利用簇状规则间隔短回文重复序列(CRISPR)/CRISPR相关蛋白9基因组编辑策略敲除SlORRM4的表达，通过降低呼吸速率和乙烯产生来延迟番茄果实的成熟。此外，当SlORRM4被敲除时，许多与果实成熟和线粒体功能相关的基因的表达发生了显著变化。此外，SLORRM4功能的丧失显著减少了许多线粒体转录本的RNA编辑，导致对线粒体复合体组装至关重要的一些核心亚单位(即Nad3、Cytc1和COX II)的低水平表达。综上所述，这些结果表明SLORRM4参与了成熟果实转录本的RNA编辑，影响了线粒体功能和果实成熟的关键方面。
RNA editing plays a key posttranscriptional role in gene expression. Existing studies on cytidine-to-uridine RNA editing in plants have focused on maize (Zea mays), rice (Oryza sativa), and Arabidopsis (Arabidopsis thaliana). However, the importance and regulation of RNA editing in several critical agronomic processes are not well understood, a notable example of which is fruit ripening. Here, we analyzed the expression profile of 33 RNA editing factors and identified 11 putative tomato (Solanum lycopersicum) fruit ripening-related factors. A rapid virus-induced gene silencing assay indicated that the organelle RNA recognition motif-containing protein SlORRM4 affected tomato fruit ripening. Knocking out SlORRM4 expression using a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 genome editing strategy delayed tomato fruit ripening by lowering respiratory rate and ethylene production. Additionally, the expression of numerous genes associated with fruit ripening and mitochondrial functions changed significantly whenSlORRM4was knocked out. Moreover, the loss ofSlORRM4function significantly reduced RNA editing of many mitochondrial transcripts, leading to low-level expression of some core subunits that are critical for mitochondrial complex assembly (i.e. Nad3, Cytc1, and COX II). Taken together, these results indicate that SlORRM4 is involved in RNA editing of transcripts in ripening fruit that influence mitochondrial function and key aspects of fruit ripening.