Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman
Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman
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DOI：
10.1016/j.cub.2018.05.042
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发表时间：
2018-07-23
期刊：
影响因子：
9.2
通讯作者：
Ueda, Minoru
中科院分区：
文献类型：
--
作者：
Oikawa, Takaya;Ishimaru, Yasuhiro;Ueda, Minoru
自查尔斯达尔文时代以来，豆科植物昼夜节律的叶片开合就引起了科学家的注意。夜行运动是由叶底部运动细胞的交替膨胀和收缩触发的。这反过来又通过改变离子流过阴离子和钾离子通道所带来的渗透压而变得容易。然而，关键的调节离子通道和分子机制在很大程度上仍然不清楚。在这里，我们确定了拟态植物Samanea Saman中的三个关键离子通道：慢型阴离子通道SsSLAH1和SsSLAH3，以及Shaker型钾通道SPORK2。我们发现SsSLAH1的细胞特异性昼夜节律表达在夜生型叶片开放中起着关键作用。此外，SsSLAH1和SsSLAH3在屈肌(远轴)运动细胞中共表达促进了叶片的张开。我们利用SLAH1受损的大豆极大值来证实SLAH1在叶片运动中的重要性。这一“主力玩家”的发现促进了我们对夜视手术的分子理解。
The circadian leaf opening and closing (nyctinasty) of Fabaceae has attracted scientists' attention since the era of Charles Darwin. Nyctinastic movement is triggered by the alternate swelling and shrinking of motor cells at the base of the leaf. This, in turn, is facilitated by changing osmotic pressures brought about by ion flow through anion and potassium ion channels. However, key regulatory ion channels and molecular mechanisms remain largely unknown. Here, we identify three key ion channels in mimosoid tree Samanea saman: the slow-type anion channels, SsSLAH1 and SsSLAH3, and the Shaker-type potassium channel, SPORK2. We show that cell-specific circadian expression of SsSLAH1 plays a key role in nyctinastic leaf opening. In addition, SsSLAH1 co-expressed with SsSLAH3 in flexor (abaxial) motor cells promoted leaf opening. We confirm the importance of SLAH1 in leaf movement using SLAH1-impaired Glycine max. Identification of this "master player" advances our molecular understanding of nyctinasty.