A novel p64-related Cl- channel:: subcellular distribution and nephron segment-specific expression
A novel p64-related Cl- channel:: subcellular distribution and nephron segment-specific expression
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DOI：
10.1152/ajprenal.1999.276.3.f398
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发表时间：
1999-03-01
影响因子：
4.2
通讯作者：
Edwards, JC
中科院分区：
文献类型：
--
作者：
Edwards, JC
最近已经描述了几种密切相关的蛋白质，它们被认为是细胞内膜的氯离子通道。我们在此报道从人类细胞中克隆出的这个家族的一个新成员，并对其进行了特性描述。基于序列相似性，我们得出结论，这种新蛋白质是先前从大鼠脑中描述的一种名为p64H1的蛋白质的人类版本。p64H1的人类版本（huH1）是一种28.7 kDa的蛋白质，通过SDS - PAGE显示其表观分子量为31 kDa。在Northern印迹中检测到一条4.5 kb的单一转录本，并且在所检测的所有组织中都存在。该蛋白质在Panc - 1细胞中以一种细胞内囊泡模式表达，这种模式不同于内质网、液相内吞和转铁蛋白循环区室，但它确实与小窝蛋白共定位。在人肾中，huH1在近端小管细胞的顶端区域以弥漫模式高度表达。huH1在肾小球和远端肾单位中以囊泡模式表达量较少。
Several closely related proteins that have been implicated as chloride channels of intracellular membranes have recently been described. We report here the molecular cloning and characterization of a new member of this family from human cells. On the basis of sequence similarity, we conclude that this new protein represents the human version of a previously described protein from rat brain named p64H1. The human version of p64H1 (huH1) is a 28.7-kDa protein that shows an apparent molecular mass of 31 kDa by SDS-PAGE. A single 4.5-kb message is detected on Northern blots and is present in all tissues probed. The protein is expressed in an intracellular vesicular pattern in Panc-1 cells that is distinct from the endoplasmic reticulum, fluid-phase endocytic, and transferrin-recycling compartments, but which does colocalize with caveolin. In human kidney, huH1 is highly expressed in a diffuse pattern in the apical domain of proximal tubule cells. huH1 is expressed less abundantly in a vesicular pattern in glomeruli and distal nephron.