DISTRIBUTION OF GAP-JUNCTIONS IN DOG AND RAT VENTRICLE STUDIED WITH A DOUBLE-LABEL TECHNIQUE
DISTRIBUTION OF GAP-JUNCTIONS IN DOG AND RAT VENTRICLE STUDIED WITH A DOUBLE-LABEL TECHNIQUE
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DOI：
10.1016/0022-2828(92)91085-j
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发表时间：
1992-12-01
影响因子：
5
通讯作者：
SPACH, MS
中科院分区：
文献类型：
--
作者：
DOLBER, PC;BEYER, EC;SPACH, MS
为了评估狗和大鼠心室石蜡切片中间隙连接相对于心肌细胞表面的分布，用麦芽凝集素 (WGA1) 标记肌膜，并用心肌间隙连接蛋白 connexin43 的抗体标记间隙连接。 WGA 标记了所有肌细胞肌膜，包括闰盘和横管中的肌细胞肌膜。肌膜 WGA 标记经常在间隙连接位点中断，间隙连接位于肌细胞的最末端和沿相邻肌细胞的长度。小间隙连接主要出现在闰盘的褶皱横向部分；较大且有时呈带状的间隙连接在纵向部分占主导地位。闰盘的纵向部分通常延伸多个肌节长度，具有带状间隙连接和平行排列的较小间隙连接的线性阵列，覆盖连续的肌节。形态测量研究表明，在狗和大鼠的左心室外膜心肌中，带状间隙连接相对较少，并且心肌细胞较大的动物往往具有较小的间隙连接。在狗的左心室心肌外膜中，肌细胞及其较大的间隙连接都不是随机定向的。肌细胞通常有些扁平，其最大直径平行于分离，而大间隙连接最不常见平行或垂直于分离。总体而言，数据表明肌细胞的几何形状影响间隙连接的大小和分布；双标签技术非常适合进一步探索这种影响。
To assess the distribution of gap junctions in relation to the cardiac myocyte surface in paraffin sections of dog and rat ventricle, the sarcolemma was labeled with wheat germ agglutinin (WGA1) and gap junctions were labeled with antibodies to cardiac muscle gap junction protein connexin43. WGA labeled all of the myocyte sarcolemma, including that in intercalated discs and transverse tubules. Sarcolemmal WGA labeling was often interrupted at the sites of gap junctions, which were found both at the extreme ends of myocytes and along the length of adjacent myocytes. Small gap junctions predominated at plicate transverse portions of the intercalated disc; larger and sometimes ribbon-like gap junctions predominated at longitudinal portions. The longitudinal portions of the intercalated disc often extended over multiple sarcomere lengths, with ribbon-like gap junctions and linear arrays of smaller gap junctions arranged in parallel overlying successive sarcomers. Morphometric study showed that ribbon-like gap junctions were relatively infrequent in both dog and rat left ventricular epimyocardium, and that animals with larger myocytes tended to have smaller gap junctions. In dog left ventricular epimyocardium, neither myocytes nor their larger gap junctions were randomly oriented with respect to perimysial separations; myocytes were usually somewhat flattened with their maximal diameters parallel to the separations, whereas large gap junctions were least often oriented parallel or perpendicular to the separations. Overall, the data indicate that myocyte geometry influences gap junction size and distribution; the double-label technique is ideally suited for the further exploration of that influence.