Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains:: towards functional genomics of microbial pathogens
Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains:: towards functional genomics of microbial pathogens
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DOI：
10.1046/j.1365-2958.1999.01549.x
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发表时间：
1999-09-01
影响因子：
3.6
通讯作者：
Kaufmann, SHE
中科院分区：
文献类型：
--
作者：
Jungblut, PR;Schaible, UE;Kaufmann, SHE
1993年，世界卫生组织宣布结核病为全球紧急情况，因为每年有800万新病例。已对致病微生物结核分枝杆菌菌株H37 Rv的全基因组进行了测序，该菌株包含3924个基因。我们比较了两个非强毒疫苗株M。牛BCG（芝加哥和哥本哈根）与结核分枝杆菌的两种强毒株（H37 Rv和Erdman）进行比较，以鉴定对疫苗、诊断和治疗的开发有价值的蛋白候选物。采用非平衡pH梯度电泳（NEPHGE）结合SDS-PAGE的双向电泳（2-DE）技术对分离的分枝杆菌进行分析。通过质谱鉴定不同的和特征性的蛋白质，并将其引入动态2-DE数据库（http：//www.mpiibberlin.mpg.de/2D-PAGE）。分枝杆菌细胞蛋白或培养上清的银染2-DE图谱分别包含1800或800个斑点，从中鉴定出263个。其中，54个属于培养上清液，16个和25个蛋白质在强度或位置上与M.结核病H37 Rv和Erdman，以及H37 Rv和M. bovis BCG芝加哥，分别鉴定并分类为蛋白质类。希望分枝杆菌蛋白质组的可用性将有助于设计用于预防和治疗最大健康威胁之一结核病的新措施。
In 1993, the WHO declared tuberculosis a global emergency on the basis that there are 8 million new cases per year. The complete genome of the strain H37Rv of the causative microorganism, Mycobacterium tuberculosis, comprising 3924 genes has been sequenced. We compared the proteomes of two non-virulent vaccine strains of M. bovis BCG (Chicago and Copenhagen) with two virulent strains of M, tuberculosis (H37Rv and Erdman) to identify protein candidates of value for the development of vaccines, diagnostics and therapeutics. The mycobacterial strains were analysed by two-dimensional electrophoresis (2-DE) combining non-equilibrium pH gradient electrophoresis (NEPHGE) with SDS-PAGE. Distinct and characteristic proteins were identified by mass spectrometry and introduced into a dynamic 2-DE database (http://www.mpiibberlin.mpg.de/2D-PAGE). Silver-stained 2-DE patterns of mycobacterial cell proteins or culture supernatants contained 1800 or 800 spots, respectively, from which 263 were identified. Of these, 54 belong to the culture supernatant, Sixteen and 25 proteins differing in intensity or position between M. tuberculosis H37Rv and Erdman, and H37Rv and M. bovis BCG Chicago, respectively, were identified and categorized into protein classes. It is to be hoped that the availability of the mycobacterial proteome will facilitate the design of novel measures for prevention and therapy of one of the great health threats, tuberculosis.