Expression of Epstein-Barr virus nuclear antigen 2 in insect cells from a baculovirus vector.
Expression of Epstein-Barr virus nuclear antigen 2 in insect cells from a baculovirus vector.
复制标题
来自杆状病毒载体的 Epstein-Barr 病毒核抗原 2 在昆虫细胞中的表达。
DOI：
10.1016/0042-6822(91)90792-a
复制
发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
Hearing,JC
中科院分区：
文献类型：
--
作者：
Schubach,WH;Horvath,G;Spoth,B;Hearing,JC
编码EB病毒核抗原2（EBNA-2）的开放阅读框已在重组杆状病毒载体中表达。所得产物以与来自潜伏感染或转化的B细胞系的EBNA-2相同的表观分子量迁移。兔抗血清来自接种这种材料免疫沉淀EBNA-2从细胞提取物的EBV含有细胞。在昆虫细胞中获得的高水平的蛋白质表达与在使用多种启动子的许多哺乳动物细胞系中观察到的形成鲜明对比，所述启动子包括内源性EBNA-2启动子、Moloney MuLV LTR、鼠免疫球蛋白重链启动子、人巨细胞病毒立即早期启动子和腺病毒主要晚期启动子。
The open reading frame encoding the Epstein-Barr virus nuclear antigen 2 (EBNA-2) has been expressed in a recombinant baculovirus vector. The resulting product migrates with the same apparent molecular weight as EBNA-2 from latently infected or converted B cell lines. Rabbit antisera derived from the innoculation of this material immunoprecipitated EBNA-2 from cell extracts of EBV-containing cells. The high level of protein expression obtained in insect cells stands in sharp contrast to that seen in a number of mammalian cell lines using a variety of promoters including the endogenous EBNA-2 promoter, the Moloney MuLV LTR, the murine immunoglobulin heavy chain promoter, the human cytomegalovirus immediate early promoter, and the adenovirus major late promoter.