Carboxydobrachium pacificum gen. nov., sp. nov., a new anaerobic, thermophilic, CO-utilizing marine bacterium from Okinawa Trough.
Carboxydobrachium pacificum gen. nov., sp. nov., a new anaerobic, thermophilic, CO-utilizing marine bacterium from Okinawa Trough.
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太平洋羧基虾属
DOI：
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发表时间：
2001
影响因子：
2.8
通讯作者：
F. Robb
中科院分区：
文献类型：
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作者：
T. Sokolova;J. M. Gonza;lez;N. Kostrikina;N. Chernyh;T. Tourova;C. Kato;E. Bonch‐Osmolovskaya;F. Robb
从冲绳海槽海底热喷口分离到一株新的厌氧、嗜热、CO利用细菌JMT。菌株JMT的细胞是不运动的细直杆，有时分支，具有革兰氏阳性类型的细胞壁，被S层包围。经常观察到三到五个细胞的链。该分离物在CO上以化学无机营养方式生长，产生等摩尔量的H2和CO2（根据方程CO+H2O->CO2+H2），在蛋白胨、酵母提取物、淀粉、纤维二糖、葡萄糖、半乳糖、果糖和丙酮酸盐上以有机营养方式生长，产生H2、乙酸盐和CO2。在50至80 ℃下观察到生长，最佳温度为70 ℃。最适pH为6.8-7.1。培养基中海盐的最佳浓度为20.5-25.5 g l（-1）。在最佳条件下，该菌的世代时间为7.1h。DNA G+C含量为33mol%。青霉素对JMT菌株的生长无抑制作用，氨苄青霉素、链霉素、卡那霉素和新霉素对JMT菌株的生长有完全抑制作用。16 S rDNA序列分析结果表明，JMT菌株属于革兰氏阳性菌芽孢杆菌-梭菌亚门中的嗜热厌氧菌系统发育群，但代表了该群的一个独立的分支。根据形态学、生理学特征和系统发育学资料，该分离物应归属于一个新属，建议命名为羧臂菌属。模式种为太平洋碳臂菌（Carboxydobrachium pacificum）;模式菌株为JMT（= DSM 12653 T）。
A new anaerobic, thermophilic, CO-utilizing marine bacterium, strain JMT, was isolated from a submarine hot vent in Okinawa Trough. Cells of strain JMT were non-motile thin straight rods, sometimes branching, with a cell wall of the Gram-positive type, surrounded with an S-layer. Chains of three to five cells were often observed. The isolate grew chemolithotrophically on CO, producing equimolar quantities of H2 and CO2 (according to the equation CO+H2O-->CO2+H2) and organotrophically on peptone, yeast extract, starch, cellobiose, glucose, galactose, fructose and pyruvate, producing H2, acetate and CO2. Growth was observed from 50 to 80 degrees C with an optimum at 70 degrees C. The optimum pH was 6.8-7.1. The optimum concentration of sea salts in the medium was 20.5-25.5 g l(-1). The generation time under optimal conditions was 7.1 h. The DNA G+C content was 33 mol %. Growth of isolate JMT was not inhibited by penicillin, but ampicillin, streptomycin, kanamycin and neomycin completely inhibited growth. The results of 16S rDNA sequence analysis revealed that strain JMT belongs to the Thermoanaerobacter phylogenetic group within the Bacillus-Clostridium subphylum of Gram-positive bacteria but represents a separate branch of this group. On the basis of morphological and physiological features and phylogenetic data, this isolate should be assigned to a new genus, for which the name Carboxydobrachium is proposed. The type species is Carboxydobrachium pacificum; the type strain is JMT (= DSM 12653T).