Endocrine communication between conceptus and mother: Placental lactogen stimulation of maternal behavior
Endocrine communication between conceptus and mother: Placental lactogen stimulation of maternal behavior
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DOI：
10.1159/000127098
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发表时间：
1996-07-01
影响因子：
4.1
通讯作者：
Mann, PE
中科院分区：
文献类型：
--
作者：
Bridges, RS;Robertson, MC;Mann, PE
在雌性大鼠中，研究了孕体通过分泌胎盘催乳素及其进入大脑在刺激母性行为发生中的可能作用。在第一项研究中，在妊娠第12-21天通过推拉灌注从大鼠采集的脑脊液（CSF）样品中检测到Nb-2淋巴瘤细胞生物测定中的显著促有丝分裂活性，与胎盘功能的建立一致。相反，在哺乳期和性腺切除的处女雌性中，CSF中不存在促有丝分裂活性。在第二项研究中，第12天妊娠样品中的促有丝分裂活性被大鼠胎盘催乳素-I（rPL-I）抗体中和71%，被rPL-I和rPL-II抗体的组合中和>90%。相反，在妊娠第21天，即产后1天，抗rPL-Ⅱ抗体降低了泌乳素的活性（>85%），但抗rPL-Ⅰ抗体没有降低泌乳素的活性，这表明CSF泌乳素中主要的催乳素是rPL-Ⅱ。在第三个实验中，通过将重组rPL-I和纯化的rPL-II直接注入类固醇致敏的未生育大鼠的大脑内侧视前区，评估胎盘分泌物的行为作用。与对照组雌性大鼠（5- 6天哺乳期）相比，rPL-I-和rPL-II-治疗组大鼠（2- 3天哺乳期）对母代寄养幼仔的哺乳期显著减少。因此，孕体通过其分泌的rPL（其显然获得对CSF的访问）帮助使怀孕雌性的大脑在妊娠结束时作出母性反应。发育中的孕体和怀孕女性之间的内分泌交流似乎是生物系统的重要组成部分，有助于建立成功的孕产妇护理。
The possible role of the conceptus in stimulating the onset of maternal behavior through its secretion of placental lactogens and their passage into the brain was investigated in female rats. In the first study, significant mitogenic activity in the Nb-2 lymphoma cell bioassay was detected in cerebrospinal fluid (CSF) samples collected by push-pull perfusion from rats on days 12-21 of pregnancy, coincident with the establishment of placental function. In contrast, mitogenic activity was absent from CSF in lactating and gonadectomized, virgin females. In a second study the mitogenic activity in day 12 pregnant samples was neutralized 71% with antibodies to rat placental lactogen-I (rPL-I) and >90% with a combination of antibodies to rPL-I plus rPL-II. In contrast, activity on day 21 of pregnancy, 1 day prepartum, was reduced by antibodies to rPL-II (>85%), but not by antibodies to rPL-I, indicating that the predominant lactogen in the CSF prepartum is rPL-II. The behavioral actions of placental secretions were assessed in the third experiment by infusing recombinant rPL-I and purified rPL-II directly into the medial preoptic area of the brain of steroid-primed, nulliparous rats. Latencies to respond maternally to foster young were significantly reduced in rPL-I- and rPL-II-treated rats (2- to 3-day latencies) when compared with latencies in control females (5- to 6-day latencies). Thus, the conceptus through its secretion of rPLs which apparently gain access to the CSF helps to prime the pregnant female's brain to respond maternally at the end of gestation. This endocrine communication between the developing conceptus and pregnant female appears to be an important part of the biological system which helps to establish successful maternal care.