Lipopolysaccharide-induced blood brain barrier permeability is enhanced by alpha-synuclein expression.
Lipopolysaccharide-induced blood brain barrier permeability is enhanced by alpha-synuclein expression.
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DOI：
10.1016/j.neulet.2013.06.058
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发表时间：
2013-09-13
影响因子：
2.5
通讯作者：
Murphy EJ
中科院分区：
文献类型：
--
作者：
Jangula A;Murphy EJ
由于α-突触核蛋白（Snca）参与神经炎症反应，我们确定其表达是否改变血脑屏障（BBB）通透性。为了诱导增加的BBB通透性，将Snca基因消融（KO）和野生型（WT）小鼠注射（i. p.）脂多糖（LPS）。为了评估BBB渗透性的变化，将伊文思蓝注射（i. p.）并使用荧光光谱法评估向脑中的外渗。相对于未处理的小鼠，WT小鼠在注射LPS后1、3和6小时具有BBB渗透性的显著增加。与WT小鼠相反，LPS在KO小鼠中未诱导BBB通透性的时间依赖性变化。虽然脑水肿与血脑屏障通透性增加有关，但两组间水肿无显著差异。这些结果表明，Snca表达与BBB响应于LPS的反应性开放增加相关。
Because α-synuclein (Snca) is involved in neuroinflammatory response, we determined if its expression altered blood-brain barrier (BBB) permeability. To induce increased BBB permeability, Snca gene-ablated (KO) and wild-type (WT) mice were injected (i.p.) with lipopolysaccharide (LPS). To assess changes in BBB permeability, Evans blue was injected (i.p.) and extravasation into the brain assessed using fluorescence spectroscopy. WT mice had a significant increase in BBB permeability at 1, 3, and 6 hours post-injection of LPS relative to untreated mice. Contrary to WT mice, LPS did not induce a time-dependent change in BBB permeability in KO mice. Although brain edema is associated with increased BBB permeability, no significant difference in edema was found between groups. These results show that Snca expression is associated with increased reactive opening of the BBB in response to LPS.
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