Influence of estradiol, stress, and 5-HT2A agonist treatment on brain-derived neurotrophic factor expression in female rats
Influence of estradiol, stress, and 5-HT2A agonist treatment on brain-derived neurotrophic factor expression in female rats
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DOI：
10.1016/s0006-3223(03)00236-1
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发表时间：
2003-07-01
影响因子：
10.6
通讯作者：
Duman, RS
中科院分区：
文献类型：
--
作者：
Cavus, I;Duman, RS
背景：雌二醇影响神经元可塑性、情绪和认知。我们检查了发情周期、急性和慢性雌二醇治疗对雌性大鼠海马和皮质中 BDNF mRNA 表达的影响。还探讨了 5-HT2A 受体和应激对 BDNF mRNA 调节的作用。方法：采用原位杂交技术测定急性和慢性卵巢切除 (OVX) 雌性大鼠发情前期和发情期以及急性和慢性雌二醇治疗后的 BDNF mRNA 水平。一些大鼠预先接受5-HT2A激动剂和拮抗剂治疗，另一组接受两小时的固定应激。结果：发情期间齿状回和内侧前额叶皮质中的BDNF mRNA水平下降，此时雌二醇水平最高。急性雌二醇治疗可降低急性 OVX 大鼠海马 BDNF mRNA，但急性或慢性雌二醇对慢性 OVX 大鼠均无影响。雌二醇预处理减少了 5-HT2A 受体介导的皮质 BDNF mRNA 上调，但不影响应激诱导的齿状回 BDNF mRNA 下调。结论：雌二醇治疗的持续时间和卵巢激素剥夺的持续时间是调节 BDNF 合成的重要因素，也可能是影响 BDNF 功能结果的重要因素。 雌激素治疗。 Biol Psychiatry 2003；54:59-69 (C) 2003 生物精神病学协会。
Background: Estradiol affects neuronal plasticity, mood, and cognition. We examined the effects of the estrous cycle, acute and chronic estradiol treatments on BDNF mRNA expression in the hippocampus and cortex of female rats. The roles of 5-HT2A receptors and of stress on the BDNF mRNA regulation were also explored.Methods: BDNF mRNA levels were measured using in situ hybridization at proestrus and estrus, and following acute and chronic estradiol treatment of acutely and chronically ovariectomized (OVX) female rats. Some rats were pre-treated with 5-HT2A agonist and antagonist, and another group was subjected to two-hour immobilization stress.Results: BDNF mRNA levels in the dentate gyrus and the medial prefrontal cortex were decreased during estrus, when estradiol levels are highest. Acute estradiol treatment decreased hippocampal BDNF mRNA in acutely OVX rats, but neither acute nor chronic estradiol had effect in chronically OVX rats. Estradiol pretreatment reduced the 5-HT2A receptor-mediated cortical upregulation in BDNF mRNA and did not effect the stress-induced down-regulation of BDNF mRNA in the dentate gyrus.Conclusions: The duration of the estradiol treatment and the duration of the ovarian hormone deprivation are important factors in the regulation of BDNF synthesis and possibly in the functional outcome of estrogen treatment. Biol Psychiatry 2003;54:59-69 (C) 2003 Society of Biological Psychiatry.