Clickable Nucleic Acids: Sequence- Controlled Periodic Copolymer/ Oligomer Synthesis by Orthogonal Thiol-X Reactions
Clickable Nucleic Acids: Sequence- Controlled Periodic Copolymer/ Oligomer Synthesis by Orthogonal Thiol-X Reactions
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DOI：
10.1002/anie.201506711
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发表时间：
2015-11-23
影响因子：
16.6
通讯作者：
Bowman, Christopher N.
中科院分区：
文献类型：
--
作者：
Xi, Weixian;Pattanayak, Sankha;Bowman, Christopher N.
合成聚合物方法通常缺乏控制一级序列的能力，序列控制被称为圣杯。现在，两个点击化学反应被组合以在简单的聚合反应中形成含核碱基的序列控制的聚合物。使用两种不同的方法来形成这些点击核酸（CNA）聚合物。这些方法采用硫醇-烯和硫醇-迈克尔反应以形成单个核碱基的均聚物（例如，聚（A）（n））或特定重复核碱基序列的均聚物（例如，poly（ATC）（n））。此外，将单官能硫醇封端的聚合物掺入聚合体系中使得能够在单个反应容器中制备多嵌段共聚物;可以通过化学计量比和/或单体官能度来调节二嵌段共聚物的长度。这些聚合物也用于有机凝胶形成，其中互补的CNA基聚合物形成可逆的交联。
Synthetic polymer approaches generally lack the ability to control the primary sequence, with sequence control referred to as the holy grail. Two click chemistry reactions were now combined to form nucleobase-containing sequence-controlled polymers in simple polymerization reactions. Two distinct approaches are used to form these click nucleic acid (CNA) polymers. These approaches employ thiol-ene and thiol-Michael reactions to form homopolymers of a single nucleobase (e.g., poly(A)(n)) or homopolymers of specific repeating nucleobase sequences (e.g., poly(ATC)(n)). Further-more, the incorporation of monofunctional thiol-terminated polymers into the polymerization system enables the preparation of multiblock copolymers in a single reaction vessel; the length of the diblock copolymer can be tuned by the stoichiometric ratio and/or the monomer functionality. These polymers are also used for organogel formation where complementary CNA-based polymers form reversible cross-links.