Antiinflammatory and ROS Suppressive Effects of the Addition of Fiber to a High-Fat High-Calorie Meal
Antiinflammatory and ROS Suppressive Effects of the Addition of Fiber to a High-Fat High-Calorie Meal
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DOI：
10.1210/jc.2016-2669
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发表时间：
2017-03-01
影响因子：
5.8
通讯作者：
Dandona, Paresh
中科院分区：
文献类型：
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作者：
Ghanim, Husam;Batra, Manav;Dandona, Paresh
背景：纤维摄入与减少心血管事件和糖尿病的发生有关。目的：研究在高脂肪、高热量(HFHC)餐中添加纤维是否能预防由HFHC餐引起的促炎改变。设计：10名正常禁食受试者在两次单独的治疗中，分别摄入或不添加30克不溶性膳食纤维的HFHC餐。结果：与单纯膳食相比，添加纤维的HFHC膳食显著降低了前90分钟的血糖漂移，增加了胰岛素和C肽的分泌。HFHC膳食诱导脂多糖(LPS)浓度、单核细胞活性氧生成以及白介素1β、肿瘤坏死因子α、Toll样受体4和CD14的表达增加。纤维的加入阻止了内毒素的增加，并显著降低了ROS的产生和IL-1β、TNF-α、TLR-4和CD14的表达。此外，膳食还增加了细胞因子信号转导抑制因子(SOCS)-3和蛋白酪氨酸磷酸酶1B(PTP-1B)信使RNA和蛋白质的水平，而添加纤维则对其有抑制作用。结论：在促炎症HFHC膳食中添加纤维具有良好的抗炎和代谢作用。因此，美国心脏协会膳食的纤维含量可能有助于其非炎症性。如果膳食纤维的这些作用在长期摄入后持续下去，它们可能有助于纤维在预防胰岛素抵抗、2型糖尿病和动脉粥样硬化方面的已知益处。
Background: Fiber intake is associated with a reduction in the occurrence of cardiovascular events and diabetes.Objective: To investigate whether the addition of fiber to a high-fat, high-calorie (HFHC) meal prevents proinflammatory changes induced by the HFHC meal. Design: Ten normal fasting subjects consumed an HFHC meal with or without an additional 30 g of insoluble dietary fiber on 2 separate visits. Blood samples were collected over 5 hours, and mononuclear cells (MNCs) were isolated.Results: Fiber addition to the HFHC meal significantly lowered glucose excursion in the first 90 minutes and increased insulin and C-peptide secretion throughout the 5-hour follow-up period compared with the meal alone. The HFHC meal induced increases in lipopolysaccharide (LPS) concentrations, MNC reactive oxygen species generation, and the expression of interleukin (IL)-1 beta, tumor necrosis factor a (TNF-alpha), Toll-like receptor (TLR)-4, and CD14. The addition of fiber prevented an increase in LPS and significantly reduced the increases in ROS generation and the expression of IL-1 beta, TNF-alpha, TLR-4, and CD14. In addition, the meal increased Suppressor of cytokine signaling (SOCS)-3 and protein tyrosine phosphatase 1B (PTP-1B) messenger RNA and protein levels, which were inhibited when fiber was added.Conclusions: The addition of fiber to a proinflammatory HFHC meal had beneficial anti-inflammatory and metabolic effects. Thus, the fiber content of the American Heart Association meal may contribute to its noninflammatory nature. If these actions of dietary fiber are sustained following long-term intake, they may contribute to fiber's known benefits in the prevention of insulin resistance, type 2 diabetes, and atherosclerosis.