R-Plasmid-mediated Chromosome Mobilization in the Facultative Methylotroph Pseudomonas AMI
R-Plasmid-mediated Chromosome Mobilization in the Facultative Methylotroph Pseudomonas AMI
复制标题
兼性甲基营养假单胞菌 AMI 中 R 质粒介导的染色体动员
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
P. Goodwin
中科院分区：
文献类型：
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作者：
P. K. Tatra;P. Goodwin
摘要：利用广泛寄主范围的R68.45质粒，对兼性甲硫氨酸假单胞菌AM1的染色体基因进行了动员。编码C1代谢的基因以每个供体细胞10-5~10-4的频率转移。编码对环丝氨酸、磷霉素和链霉素的抗性的基因与甲醇脱氢酶基因连锁，而硫胺素营养缺乏症的标记不连锁。
SUMMARY: Chromosomal genes of the facultative methylotroph, Pseudomonas AM1, have been mobilized using the broad host-range plasmid, R68.45. Genes coding for C1 metabolism were transferred at a frequency of 10-5 to 10-4 per donor cell. The genes coding for resistance to cycloserine, phosphonomycin and streptomycin are linked to the methanol dehydrogenase gene, while the marker for thiamin auxotrophy is not.