Pulmonary toxicity of well-dispersed single-wall carbon nanotubes after inhalation
Pulmonary toxicity of well-dispersed single-wall carbon nanotubes after inhalation
复制标题
DOI：
10.3109/17435390.2011.620719
复制
发表时间：
2012-11-01
期刊：
影响因子：
5
通讯作者：
Tanaka, Isamu
中科院分区：
文献类型：
--
作者：
Morimoto, Yasuo;Hirohashi, Masami;Tanaka, Isamu
通过超声处理使单壁碳纳米管（SWCNT）良好分散，以进行吸入研究。单壁碳纳米管是使用加压雾化器和单壁碳纳米管液体悬浮液生成的。将Wistar大鼠暴露于分散良好的SWCNT(束直径：0.2μm；束长度：0.7μm)4周。 SWCNT 的低质量浓度和高质量浓度分别为 0.03 +/- 0.003 和 0.13 +/- 0.03 mg/m(3)。暴露结束后3天、1个月和3个月处死大鼠。在高浓度和低浓度暴露组中，支气管肺泡灌洗液（BALF）中的总细胞或中性粒细胞计数没有增加，或者肺部或BALF中细胞因子诱导的中性粒细胞趋化剂浓度没有增加。在整个观察期间，任一暴露组均未观察到中性粒细胞的肺部浸润。在本研究的条件下，分散良好的单壁碳纳米管不会诱发肺部中性粒细胞炎症。
Single-wall carbon nanotubes (SWCNTs) were well-dispersed by ultrasonication to conduct an inhalation study. SWCNTs were generated using a pressurised nebuliser with liquid suspension of SWCNTs. Wistar rats were exposed to the well-dispersed SWCNT (diameter of bundle: 0.2 mu m; length of bundle: 0.7 mu m) for 4 weeks. The low and high mass concentrations of SWCNTs were 0.03 +/- 0.003 and 0.13 +/- 0.03 mg/m(3), respectively. The rats were sacrificed at 3 days, 1 month, and 3 months after the end of exposure. There were no increases of total cell or neutrophil counts in the bronchoalveolar lavage fluid (BALF), or the concentration of cytokine-induced neutrophil chemoattractant in the lungs or BALF in both the high and low concentration-exposed groups. Pulmonary infiltration of neutrophils was not observed in either exposed group throughout the observation period. Well-dispersed SWCNT did not induce neutrophil inflammation in the lung under the conditions in the present study.